OBJECTIVES We investigated the genotoxic effects of 40-59 nm silver nanoparticles (Ag-NPs) by bacterial reverse mutation assay (Ames test), in vitro comet assay and micronucleus (MN) assay. In particular, we directly compared the effect of cytochalasin B (cytoB) and rat liver homogenate (S9 mix) in the formation of MN by Ag-NPs. METHODS Before testing, we confirmed that Ag-NPs were completely...

OBJECTIVE To examine the role of antiretroviral drugs (ART), HIV-related and traditional risk factors on the incidence of chronic kidney disease (CKD) in HIV-infected patients. DESIGN Prospective hospital-based cohort of HIV-infected patients from 2004 to 2012. METHODS CKD was defined using MDRD equation as an estimated glomerular filtration rate (eGFR) less than 60 ml/mn/1.73 m(2) at 2 con...

The aim of the present study was to characterize the action of the neurotransmitter, NO, on rat myenteric neurons. A NO donor such as GEA 3162 (10 moll) induced an increase in the intracellular Ca concentration as indicated by an increase in the fura-2 ratio in ganglia loaded with this Ca-sensitive fluorescent dye. The effect of GEA 3162 was strongly reduced in the absence of extracellular Ca s...

a significant portion of manganese (mn) chemical fertilizers applied to calcareous soils is not absorbed by the first crop and may, therefore, affect the growth as well as chemical composition of the subsequent crops, this being called the residual effect of mn, soil tests may be employed to predict such effects. the present experiment was conducted to study the residual effects of manganese su...

1. The maximum capacity of the bovine liver to excrete manganese in bile was determined in three Friesian-cross steers surgically prepared to allow bile flow-rate to be measured and samples collected. Plasma Mn concentrations were increased by infusing manganese chloride solutions into a jugular vein and the biliary excretion rates of Mn, copper, zinc and iron were measured. 2. The maximum capa...

a pot experiment in randomized complete blocks design (rcbd) with six treatments in four replications was conducted to study the effect of water stress on morphological characteristics and essential oil content of improved basil (ocimum basilicum c.v. keshkeni luvelou). the irrigation treatments for induction of water stress were: a: (125 ml/ 12 h), b: (250 ml/ 24 h), c: (250 ml/ 48 h), d: (500...

Saxagliptin (SAX) is an oral drug that a hypoglycemic (between anti-diabetic agent) dipeptidyl peptidase-4 inhibitor. In this study, cytotoxic, genotoxic effects and DNA damage of SAX on human lymphocytes were investigated. For purpose, Single Cell Gel Electrophoresis (SCGE), Micronucleus (MN) Mitotic Index (MI) tests used. Based the daily doses SAX, 0.017, 0.035, 0.07, 0.14 µg/mL concentratio...

KIR3DL1 is an inhibitory HLA-B receptor of human NK and T cells that exhibits genetic and phenotypic polymorphism. KIR3DL1*004, a common allotype, cannot be detected on the surface of PBLs using the KIR3DL1-specific Ab DX9. The nature of this phenotype was investigated through comparison of 3DL1*004 with 3DL1*002, an allele giving high DX9 binding to cell surfaces. Analysis of Jurkat T cell tra...