MicroRNAs (miRNAs) are a class of short, non-coding RNA that play regulatory roles in a wide variety of biological processes, such as plant growth and abiotic stress responses. Although several computational tools have been developed to identify primary miRNAs and precursor miRNAs (pre-miRNAs), very few provide the functionality of locating mature miRNAs within plant pre-miRNAs. This manuscript...

MicroRNAs (miRNAs) play an important role in development and regulate the expression of many animal genes by post-transcriptional gene silencing. Here we describe the cloning and expression of new miRNAs from zebrafish. By high-throughput sequencing of small-RNA cDNA libraries from 5-day-old zebrafish larvae and adult zebrafish brain we found 139 known miRNAs and 66 new miRNAs. For 65 known miR...

The precise regulation of microRNA (miRNA) transcription and processing is important for eukaryotic development. Plant miRNAs are first transcribed as stem-loop primary miRNAs (pri-miRNAs) by RNA polymerase II,then cleaved in the nucleus into mature miRNAs by Dicer-like 1 (DCL1). We identified a cycling DOF transcription factor, CDF2, which interacts with DCL1 and regulates the accumulation of ...

Background: Human consumption of food and herbal medicines supplies the body with not only nutrients but also associated biologically active compounds, such as exogenic messenger RNA (mRNA) inhibitory RNAs (miRNAs). Therefore, it seems important to establish which maize wheat miRNAs can enter human evaluate their possible actions binding features mRNA genes. It is required determine whether mos...

Background: Intrahepatic covalently closed circular DNA (cccDNA) plays a critical role in the life cycle of hepatitis B virus (HBV). Growing evidence suggests that microRNAs (miRNAs) may regulate cccDNA expression and contribute to natural history chronic (CHB). Objectives: This study aimed investigate potential miRNA-mRNA regulatory axes intrahepatic CHB-GZ patients identify new therapeutic ta...

Introduction In this study, we validated the changes in microRNA (miRNA) expression hypoxic human kidney 2 (HK2) cell-derived extracellular vesicles (EVs). Additionally, investigated mechanism by which miRNA that are derived from EVs alleviated renal ischemia-reperfusion (I/R) injury. Material and methods HK2 cells were treated a chamber (1% O2) for 12 h.EVs obtained as supernatant ultracentrif...

MicroRNAs (miRNAs) in blood plasma are stable under high levels of ribonuclease activity and could function in tissue-to-tissue communication, suggesting that they may have distinctive structural characteristics compared with non-circulating miRNAs. In this study, the expression of miRNAs in horse plasma and their characteristic nucleotide composition were examined and compared with non-plasma ...