The probe 29C1 detects a hypervariable locus 18kb from the telomere of the human X and Y chromosomes, in the pseudoautosomal region. Here we report that hypervariability of fragments containing this sequence in the human population arises by loss or gain of a 31 base pair GC rich repeat. Labelled 29C1 does not detect a DNA fingerprint at low stringency, though the consensus repeat sequence does...

Clinical and environmental Vibrio cholerae organisms collected from February 2004 through April 2005 were systematically isolated from 2 rural Bangladeshi locales. Their genetic relatedness was evaluated at 5 loci that contained a variable number of tandem repeats (VNTR). The observed minimal overlap in VNTR patterns between the 2 communities was consistent with sequential, small outbreaks from...

In the present work, we investigated the phylogenetic position and phenotypic characteristics of eight yeast isolates collected from migratory birds on the island of Ustica, Italy. A phylogenetic analysis based on the D1/D2 region of the large-subunit rRNA gene showed that all isolates clustered as a single separate lineage within the Wickerhamomyces clade. They exhibited distinct morphological...

The German Satellite CHAMP (Challenging Minisatellite Payload) has the unique ability to measure the surface forces present. The total density can be easily derived from this measurement, with the help of models of solar radiation pressure and Earth’s albedo. This total density will be compared with the total density from two models, MSIS-86 (Hedin, 1987) and DTM-78 (Barlier et al., 1977). The ...

We used the method of Information Decomposition developed by us to identify the latent dinucleotide periodicity regions in bacterial genomes. The number of potential minisatellite sequences obtained at high level of statistical significance was 454. Then we classified the periodicity matrices and obtained 45 classes. We used the other new method developed by us--Modified Profile Analysis--to re...

Within the past decade, two reports have appeared purporting to demonstrate much greater human sensitivities to the genetic effects of radiation than are consistent with the Japanese or mouse data that I summarized earlier in this program. This presentation will review these reports, both of which are badly flawed, and then proceed to a few poignant remarks concerning the responsibilities of sc...

Minisatellite and microsatellite are short tandemly repetitive sequences dispersed in eukaryotic genomes, many of which are highly polymorphic due to copy number variation of the repeats. Because mutation changes copy numbers of the repeat sequences in a generalized stepwise fashion, stepwise mutation models are widely used for studying the dynamics of these loci. We propose a minimum chi-squar...

There are a number of studies that show radiation can cause heritable mutations in the offspring of irradiated organisms. These "germ-line mutations" have been shown to occur in unique sequences of DNA called "minisatellite loci". The high frequencies of spontaneous and induced mutations at minisatellite loci allow mutation induction to be measured at low doses of exposure in a small population...

Pig-) The amount of polymorphic frag| merits in a DNA fingerprint d e ^ |-f I 1» pends on characteristics of the 0.8* individual's genome, restriction enzymes and minisatellite B [U l I probes used. In addition h ow0-8* i.»% ever, a high resolution of the DNA fragments during gel electrophoresis is essential. Therefore the following simple method was applied: Instead of using an agarose gel of ...

Radioactivity is known to induce tumors, chromosome lesions, and minisatellite length mutations, but its effects on the DNA sequence have not previously been studied. A coastal peninsula in Kerala (India) contains the world's highest level of natural radioactivity in a densely populated area, offering an opportunity to characterize radiation-associated DNA mutations. We sampled 248 pedigrees (9...