Purpose We previously found that the histone methyltransferase suppressor of variegation, enhancer of zeste, trithorax and myeloid-nervy-deformed epidermal autoregulatory factor-1 domain-containing protein 3 (SMYD3) is a potential independent predictive factor or prognostic factor for overall survival in gastric cancer patients, but its roles seem to differ from those in other cancers. Therefor...

The leukocyte migration inhibition assay was used to detect cell-mediated immune reaction of hepatoma patients. Extraction of 3 pairs of human hepatoma tissue and normal liver tissue was made by 3 M KCI solution, and soluble hepatoma antigens were used to test against leukocytes of 18 hepatoma patients by the leukocyte migration inhibition assay. In all, 25 tests were performed; most of them we...

BACKGROUND Bo-Gan-Whan (BGH), a Korean polyherbal medicine, is used as a hepatoprotective drug. It has six natural sources, and has been demonstrated to have anti-oxidative, anti-cancer, and anti-inflammatory properties; however, its effect on vascular diseases remains unclear. METHODS Cell viability and proliferation assays were employed using an EZ-Cytox Cell Viability Assay Kit. Platelet-d...

PURPOSE To investigate changes in cell proliferation and migration of human bone marrow-derived mesenchymal stromal cells (hMSCs) in glioma environment, in order to assess the tumorigenic risk of hMSCs in the clinical application to treat human gliomas. METHODS hMSCs were obtained from normal adult persons and identified by their morphological characteristics and test of their stemness. The U...

Migration and invasiveness are phenotypic characteristics of cells that contribute to physiological processes, such as wound healing or embryogenesis and they are involved in serious pathological processes, namely in tumor cell metastasis. Availability of methods for studying migration and invasiveness of the cells is important for understanding molecular basis of these processes. In the case o...

Objectives Vascular smooth muscle cells (VSMCs) play a key role in the pathogenesis of diabetic vascular disease. Our current study sought to explore the effects of tanshinone IIA on the proliferation and migration of VSMCs induced by advanced glycation end products (AGEs). Materials and Methods In this study, we examined the effects of tanshinone IIA by cell proliferation assay and cell migr...

c-Src activates Ras-MAPK/ERK signaling pathway and regulates cell migration, while trihydrophobin 1 (TH1) inhibits MAPK/ERK activation and cell migration through interaction with A-Raf and PAK1 and inhibiting their kinase activities. Here we show that c-Src interacts with TH1 by GST-pull down assay, coimmunoprecipitation and confocal microscopy assay. The interaction leads to phosphorylation of...

objective(s):eukaryotic translation initiation factor 4e (eif4e) is overexpressed in cervical cancer (cc). however, the molecular mechanisms are unclear. this study aimed to investigate the molecular mechanism of eif4e gene overexpression in cc. materials andmethods:the human papillomavirus (hpv) type 18 e7 and eif4e mrnas were measured following knock down or overexpression of e7 gene by rt-pc...

Introduction Papillary thyroid carcinoma (PTC) is the most common type of malignancy with increased incidence rapidly occurring in countries. The study aimed to investigate antitumor effects combination 5-FU and Apatinib on PTC. Material methods TPC-1 SW579 cells were treated 5-FU, Apatinib, or a Apatinib. Cell viability was assessed by CCK-8 assay. Edu staining colony formation used evaluate c...


 Purpose: To investigate the regulatory effect and molecular mechanism of miR-143-5p in colorectal cancer (CRC) progression.
 Methods: Expression CRC cell lines SW620 HCT116 was determined by quantitative real-time polymerase chain reaction (qRT-PCR). Stable overexpression mediated lentivirus. The effects on proliferation, migration, invasion, epithelial- mesenchymal transition (EMT)...