PREMISE OF THE STUDY Microsatellite primers were developed to characterize and evaluate patterns of genetic diversity and structure in the endangered Mediterranean shrub Ziziphus lotus (Rhamnaceae). METHODS AND RESULTS Twenty microsatellite primers were developed for Z. lotus, of which 14 were polymorphic. We evaluated microsatellite polymorphism in 97 specimens from 18 Spanish and seven Moro...

The possible effects of a selected locus on a closely linked microsatellite locus are discussed and analyzed in terms of coalescent theory and models of the mutation process. Background selection caused by recurrent deleterious mutations will reduce the variance of allele size at a microsatellite locus. The occasional substitution of advantageous alleles (genetic hitchhiking) will also reduce t...

This study aims to provide a basis for the evaluation, protection, and utilization of the genetic resources of Chinese Yellow Quails by analyzing the polymorphism of these quail groups with nine microsatellite markers using the polyacrylamide gel electrophoresis. A total of 48 alleles were identified from nine microsatellite markers, with an average of 5.3 alleles per marker. The average hetero...

Vol. 36, No. 3 (2004) BioTechniques 409 The process of microsatellite development and profiling involves three primary steps: (i) isolating regions of genomic DNA that contain microsatellite loci; (ii) developing strategies for screening each locus, which requires designing primers for amplification, optimizing reaction conditions, and screening for variation; and (iii) genotyping sampled indiv...

Association Analysis for Important Quantitative and Morphological Traits in Cultivars Advanced Lines of Soybean (Glycine max (L.)) using Microsatellite Markers

  Background and Objectives: Hereditary nonpolyposis colorectal cancer (HNPCC) is an autosomal dominant cancer predisposition syndrome caused by germ-line mutations in DNA mismatch repair genes. Tumors arising as a result of these mutations display instability in a sequence area known as microsatellites. Studies have shown that some Bethesda markers (BAT25, BAT26) are more efficient than other...

Understanding the scale at which wild stocks of Persian sturgeon (Acipenser persicus) are genetically discrete is necessary for effective management of this commercially important species. Disomic DNA microsatellite markers are among the best tools for determining stock structure in fishes. As all sturgeon species have a polyploid ancestry of all sturgeons, most gene loci exhibit more than two ...