Background: Secondary metabolites in stevia leaves offer important therapeutic benefits. The germination problems of seeds and the high heterogeneity resulting populations make micropropagation preferred tool for obtaining a large number plants short time. Until now, studies have focused on optimizing action growth regulators improvement micropropagated plantlets. In this project, we study alte...

An interaction study was performed with mycorrhizal plants of the grapevine rootstock Richter 110 (Vitis berlandieri Planch × Vitis rupestris Scheele) and the root pathogenic fungus Armillaria mellea (Vahl:Fr.) P. Kumm using an autotrophic in vitro culture system. Micropropagated plantlets were transferred to Petri plates with MSR medium lacking sugar and vitamins. Inocula of Glomus intraradice...

Shoot tips from actively growing, greenhouse maintained plants of Kalanchoe blossfeldiana were cultured in vitro for shoot proliferation and root initiation on Murashige and Skoog (MS) basal medium supplemented with NAA and BA, both in concentrations of 0.00, 0.50, 1.00 and 2.00 mg l-1. Results showed that the maximum plantlets height (7.012 cm), node number (4.516), root number (8.860) and roo...

Increased planting density, different types of thinning management, and the use beneficial microorganisms have been adopted as strategic tools in effort to increase crop yields a sustainable manner. This investigation evaluated growth production ‘Prata Anã Gorutuba’ banana ( Musa spp., AAB) under plant densities during two cycles semiarid region Minas Gerais, Brazil. Micropropagated plantlets w...

An in vitro plant regeneration protocol was successfully established in Aphyllorchis Montana , a saprophytic achlorophyllous orchid by culturing immature seeds. Among the six basal media evaluated for seed germination, BM-TM medium was found to be the best followed by KC medium. After 40 days, all the media turned brown and the growths of the protocorms were arrested. Activated charcoal, 1 g/l ...

In micropropagation and in vitro conservation various nutrient media have been used for regeneration of citrus rootstocks. this respect, the present study was conducted to define, among three culture media, one common use (MS) two developed arboreal species (RMAN WPM), best formulation establishment, propagation vitro. Plantlets from 10 rootstocks grown were micropropagated conserved 18 months,...

Matthiola incana (Brassicaceae) is an important ornamental plant, which is used as cut flower, pot plant, and landscape. Traditionally, it is propagated via seeds, but interest is given in vegetative propagation of parental lines as well as superior single plants. Micropropagation by organogenesis is an efficient in vitro propagation method for Matthiola incana. Starting from seeds, apical bud ...

Banana micropropagation for obtaining free-virus plants frequently provokes somaclonal variation that could increase useful genetic variability in this asexually propagated crop. Both exploring the cycle of vitro culture which occurs and amount generated polymorphism, are necessary. In work, preliminary results during early cycles banana reported. Four randomly selected regenerated from fifth t...