Several Bacillus strains belonging to the B. subtilis/amyloliquefaciens group isolated from plant-pathogen-infested soil possess plant-growth-promoting activity [Krebs, B. et al. (1998) J Plant Dis Prot 105, 181-197]. Three out of the four strains investigated were identified as B. amyloliquefaciens and were able to degrade extracellular phytate (myo-inositol hexakisphosphate). The highest extr...

In this study, an extracellular alkali-thermostable phytase producing bacteria, Bacillus subtilis B.S.46, were isolated and molecularly identified using 16S rRNA sequencing. Response surface methodology was applied to study the interaction effects of assay conditions to obtain optimum value for maximizing phytase activity. The optimization resulted in 137% (4.627 U/mL) increase in phytase activ...

Two experiments were conducted to study the effect of phytase supplementation to lowphosphorus starter and finisher broiler diets on mineral utilization and growth performance. All diets in this study were isonitrogenous, isocaloric and corn-soybean diets. In Experiment 1, four replicate groups of 15 commercial broiler chicks, one-day old, were randomly assigned to each of two dietary treatment...

Three experiments were conducted to evaluate the effect of supplementing phytase and xylanase on nutrient digestibility and performance of growing pigs fed wheat-based diets. In Exp. 1, 10 diets were fed to 60 pigs from 20 to 60 kg of BW to determine the effect of combining phytase and xylanase on apparent total tract digestibility (ATTD) of nutrients and growth performance. The 10 diets includ...

The physical and chemical properties of six crude phytase preparations were compared. Four of these enzymes (Aspergillus A, Aspergillus R, Peniophora and Aspergillus T) were produced at commercial scale for the use as feed additives while the other two (E. coli and Bacillus) were produced at laboratory scale. The encoding genes of the enzymes were from different microbial origins (4 of fungal o...

A study was conducted to determine the efficacy of phytase, an enzymic cocktail, and a waste Aspergillus niger mycelium to hydrolyze phytate present in corn-soybean meal diets. One hundred turkey poults were assigned to dietary treatments for 2 wk (days 7 to 21). Dietary treatments included: 1) NRC (1994) diet (NRC), with recommended concentration of 0.6% available P (aP) and 1.2% Ca; 2) Phytas...

Forty-eight pigs (barrows:gilts, 1:1) with an average initial weight of 9.4 kg were used in a 2 x 2 factorial experiment to determine the influence of dietary phytase (0 or 1,500 phytase units/kg) and zinc (0 or 100 mg/kg) supplementation of a corn-soybean meal diet on the utilization of P, Ca, Cu, Mg, Mn, and Zn. After a 21-d growth experiment, feed was withheld for 24 h and blood was collecte...

The residual phytase activity, phytic acid P content, and microstructure of the digesta along the gastrointestinal tract (GIT) of broiler chickens fed a corn expressing microbial phytase was studied in a 14-d experiment. The phytase activity of the corn expressing phytase (CBP) was determined to be 660 phytase units/g and was incorporated into broiler diets at varying rates. One hundred forty-f...

A study was conducted to evaluate the effects of dietary supplementation of phytase B (product of the Aspergillus niger phyB gene expressed in Trichoderma reesei) on feed intake, laying performance, eggshell quality, and on phosphorus and calcium balance in laying hens. Seventy-two, 40 weeks old Hy Line Brown hens were fed for 14 weeks the following four phosphorus-deficient (0.12% nonphytate p...