نتایج جستجو برای: metaphase

تعداد نتایج: 5816  

Journal: :Zootaxa 2015
Harbhajan Kaur Kanu Sharma

Regardless of rich biodiversity, cytogenetic reports on Pentatomidae refer to less than 400 species. This paper reports, for the first time, course of meiosis of three species of subfamily Pentatominae viz., Tolumnia antennata Distant, Cahara jugatoria (Lethierry) and Cahara confusa (Distant). Species-specific variations observed with respect to association pattern of X and Y during prophase, a...

1998
Ulrich K Laemmli

Introduction Various structural studies have demonstrated that the chromosomal architecture of the nucleus is highly ordered and suggest that it might be held in place by some sort of nuclear scaffold. This organization must permit dynamic movements of chromatin during activities such as enhancer looping, recombination and chromosome condensation. The mechanisms whereby nuclear order and chroma...

Journal: :American journal of medical genetics 1996
J L Spurbeck A R Zinsmeister K J Meyer S M Jalal

Consistency of optimum chromosome spreading during harvest of cytogenetic specimens remains a major concern. We have tested the idea that a precise control of the drying rate (the time with which metaphase cells dry), as fixed cell suspension is placed on a slide or an in situ culture in last fixation, may be the answer. Amniocyte and lymphocyte cultures were allowed to dry at defined combinati...

2011
Wei Wu Xiaoli Yang Charles C. Tseng

Learning human cytogenetics is important for biology education and training of clinical cytogenetics technologists. To increase the resources of metaphase spreads with different chromosomes shapes for student practice, we describe effective algorithms for such purposes to enhance our cytogenetics tutorial program.

Journal: :PLoS ONE 2008
Emmanuel Boucrot Tomas Kirchhausen

Using single cell-imaging methods we have found that the volume of adherent cells grown in culture decreases as the cells rounds when it enters mitosis. A minimal volume is reached at metaphase. Rapid volume recovery initiates before abscission as cells make the transition from metaphase to cytokinesis. These volume changes are simultaneous with the rapid surface area decrease and recovery obse...

Journal: :Genetics 1973
Y Narayanan

The phylogenetic relationships among the species of the D. robusta group were investigated by the analysis of chromosomal differences. Six of the ten known members of the D. robusta group were available for the study: D. colorata and D. robusta from the United States, and D. sordidula, D. pseudosordidula, D. lacertosa, and D. moriwakii from Japan. Analysis of the metaphase chromosomes from larv...

M. Pourkazemi, S. Bakhshalizadeh S. Nazari

  Preparation of chromosome spreads and karyotype analysis in Blicca bjoerkna transcaucasica were carried out using 0.01% solution of colchicines and Phytohaemagglutinin (PHA) (20 µg g-1 body weight). The gill and kidney tissues were collected and let to stand in a hypotonic solution of 0.075 M KCl and then treated with a fixative (Carnoy's solution) in three steps. The chromosomes spreads were...

M. Pourkazemi, S. Bakhshalizadeh, S. Nazari,

  Preparation of chromosome spreads and karyotype analysis in Blicca bjoerkna transcaucasica were carried out using 0.01% solution of colchicines and Phytohaemagglutinin (PHA) (20 µg g-1 body weight). The gill and kidney tissues were collected and let to stand in a hypotonic solution of 0.075 M KCl and then treated with a fixative (Carnoy's solution) in three steps. The chromosomes spreads were...

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