A small zinc-binding group (ZBG) library of deoxy-2-C-branched-monosaccharides, for example, 1,5-anhydroglucitols, consisting of either monodentate ligand binding carboxylic acids or bidentate ligand binding hydroxamic acids, were prepared to assess the zinc affinity of the putative metalloenzyme 2-acetamido-2-deoxy-α-D-glucopyranosyl-(1→6)-phosphatidylinositol de-N-acetylase (EC 3.5.1.89) of g...

A multiresistant Klebsiella pneumoniae isolate was taken from the blood of a 75-year-old patient with nosocomial pneumonia who developed septic shock and failed therapy with imipenem. The isolate presented an MIC of imipenem of 128 microg/ml, and the production of a metallo-beta-lactamase was confirmed by phenotypic and genotypic techniques. We here report, for the first time, the detection of ...

A procedure is described, using a freezing microtome and measurements of tissue slice volume, to obtain serial sections of the lens for analysis of components from one side of the organ to the other. The concentrations of zinc (analyzed by atomic absorption spectrophotometry) were similar in serial sections taken across the lens from the anterior to the posterior surface. The levels of the zinc...

The measurement of cellular and sub-cellular responses to chemical contaminants (referred to as biomarkers) in living organisms represents a recent tool in environmental monitoring. The review focuses on carbonic anhydrase, a ubiquitous metalloenzyme which plays key roles in a wide variety of physiological processes involving CO(2) and HCO(3)(-). In the last decade a number of studies have demo...

Carbonic anhydrase IX (CAIX) is a transmembrane metalloenzyme which upregulated in tumour cells under hypoxic conditions. CAIX expression induced by the accumulation of hypoxia-inducible factor-1α and has several downstream effects, including acidification extracellular pH, loss cellular adhesion increased cell migration. variety solid organ tumours prognostic implications. High protein marker ...