introduction: iiron oxide nanoparticles (io np) have an increasing number of biomedical applications. to date, the potential cytotoxicity of these particles remains an issue of debate. little is known about the cellular interaction or toxic effects of io np on differentiation of stem cells. the aim of the present study was to investigate the possible toxic role of different doses of io np in di...

the wharton’s jelly of the umbilical cord is believed to be a source of mesenchymal stem cells (mscs) which can be therapeutically applied in degenerative diseases. in this study, we investigated the immunomodulatory effect of umbilical cord derived- mesenchymal stem  cells (uc-mscs) and  bone  marrow-derived-mesenchymal stem  cells (bm-mscs) on differentiation, maturation, and endocytosis of m...

in this study, our aim was to produce a generation of gmp-grade adipose tissue-derived mesenchymal stem cells for clinical applications. according to our results, we fulfill to establish consistent and also reproducible current good manufacturing practice (cgmp) compliant adipose tissue-derived mesenchymal stem cells from five female donors. the isolated cells were cultured in dmem supplemented...

background: the transcription factor oct-4, is an important marker of undifferentiating level and a key regulating factor for maintenance of pluripotency in cells. establishment of an oct-4 promoter-based reporter system is an appropriate tool for monitoring the differentiation of embryonic stem cells both in vivo and in vitro. methods: in the present study, we report construction of a recombin...

multiple sclerosis (ms) is an inflammatory demyelinating and neurodegenerative disorder of  the  central  nervous  system (cns), which mainly affects  young adults. activated t lymphocytes promote the neuro-inflammatory cascade of ms by secreting pro-inflammatory cytokines and play a significant role in its pathogenesis. t  lymphocytes may trigger the inflammation, which in turn leads to axonal...

research about potential use of stem cells for the development of germ line cells in vitro had been challenged. in the present study, we reported a novel protocol consisting of cocktail growth factor addition for germ cell differentiation followed by transfection. the cells were purificated based on the expression on the cell surface of a protein. this protein is not present in normal cells of ...

auto-reactive cells-mediated immune responses are responsible for the current tissue damages during autoimmunity. accordingly, functional modulation of auto-reactive cells has been a pivotal aim in many of recent studies. in the current study, we investigated the possibility for insertion of regulatory molecules onto auto-reactive cells through exosomal nano-shuttles as a novel approach for phe...