Genetic polymorphisms of apolipoprotein E (APOE) are associated with various health conditions and diseases, such as Alzheimer's disease, cardiovascular diseases, type 2 diabetes, etc. Hence, genotyping of APOE has broad applications in biomedical research and clinical settings, particularly in the era of precision medicine. The study aimed to develop a convenient and accurate method with flexi...

Molecular dynamics simulation is used to estimate the melting point of cobalt using embedded atomic model (EAM) potential by heat until melting, void, hysteresis and interface methods. For instance, estimated temperature are 2102 K, 1944.15 1731 K 1725±25 these methods, respectively. Then, points at different pressures calculated. A graph depicting variation with pressure drawn compared availab...

The presence of the JAK2 V617F mutation is now part of clinical diagnostic algorithms, and JAK2 status is routinely assessed when BCR/ABL- chronic myeloproliferative neoplasms (MPNs) are suspected. The aim of this study was to evaluate performance of 3 screening and 1 quantitative method for JAK2 V617F detection. For the study, 43 samples (27 bone marrow aspirates and 16 peripheral blood sample...

Methicillin-resistant Staphylococcus aureus is one of the most significant pathogens associated with health care. For efficient surveillance, control and outbreak investigation, S. aureus typing is essential. A high resolution melting curve analysis was developed and evaluated for rapid identification of the most frequent spa types found in an Austrian hospital consortium covering 2,435 beds. A...

AIMS The aim of this study was to describe quantitatively the potential demethylating effects of the plant-derived compound isoliquritigenin (ILTG) on the death-associated protein kinase-1 (DAPK1) promoter region of colon cancer and leukemia cell lines. METHODS Methylation-specific real-time polymerase chain reaction-melting curve analysis (MSP-MCA) was the primary method used. A simple cytot...

Human coronaviruses HCoV-OC43, HCoV-229E, HCoV-NL63 and HCoV-HKU1 are common respiratory viruses associated with acute respiratory infection. They have a global distribution. Rapid and accurate diagnosis of HCoV infection is important for the management and treatment of hospitalized patients with HCoV infection. Here, we developed a melting curve-based multiplex RT-qPCR assay for simultaneous d...

unknown. To determine the capability of HRM to detect mosaicism, the assay was also performed using bisulfitetreated DNA from 2 AS patient samples known to be mosaic and a PWS patient sample with a putative triplication of the SNRPN promoter region on the maternally derived chromosome 15 (as determined by semiquantitative MSP, fluorescence in situ hybridization, and microsatellite analysis). Th...

Ultrahigh-pressure phase boundary between solid and liquid SiO2 is still quite unclear. Here we present predictions of silica melting curve for the multimegabar pressure regime, as obtained from first principles molecular dynamics simulations. We calculate the melting temperatures from three high pressure phases of silica (pyrite-, cotunnite-, and Fe2P-type SiO2) at different pressures using th...