Dairy cow mastitis is an important disease in the dairy industry. Different microbial species have been identified as causative agents in mastitis, and are traditionally diagnosed by bacterial culture. The objective of this study was to use metagenomic pyrosequencing of bacterial 16S rRNA genes to investigate bacterial DNA diversity in milk samples of mastitic and healthy dairy cows and compare...

The aims of the present study were (1) to evaluate the influence of using mobile (n=47) or fixed (n=45) milking machines in Azorean herds on the apparent prevalence of several milk pathogens in bulk tank milk (BTM) and (2) to determine whether separated subclinical mastitic cows can serve, in real time, as predictors of milk pathogen prevalence for the remaining animals at the herd level. The u...

Backgrounds and Aims: Mastitis is a mammalian disease which is considered important due to its potential economic damages. Trueperella pyogenesis is one of the important opportunistic pathogens of the mammary glands of cattle. This bacterium can produce acute mastitis infection in dairy cattle. In fact, this bacterium has several virulence genes which contribute to its pathogenicity. Therefore,...

The complement system helps in the direct lysis of invading pathogens and modulates phagocytic, humoral and cellular immune responses. Complement 4 is a critical component in complement activity and protection against many bacterial pathogens because it is essential to classical and lectin activation pathways. We used reverse transcription and PCR to investigate alternative splicing and e...

We examined the effective diagnostic indicator using the concanavalin A (Con A) low-affinity lactoferrin (Lf) to mastitic drying cows. The concentrations of both Lf and Con A low-affinity Lf in mammary gland secretions (MGSs) were lower than normal MGSs at the early and middle dry periods and colostrums. On the other hand, the levels of Con A low-affinity Lf in MGSs increased following the appe...

This study determined association of some morphometric characteristics of udder with mastitis in dairy cattle. For this purpose; 100 animals each negative and positive for mastitis by California Mastitis Test and bacteriology, were selected from amongst those brought for slaughter at Faisalabad abattoir. Morphometric observations on udder were recorded pre-slaughter; whereas, samples from mamma...

The aim of this study was to compare the antimicrobial activity essential oil (EO) cinnamon (Cinnamomum verum) and its major compound cinnamaldehyde against Staphylococcus aureus (ATCC 14458) bacteria isolated from mastitic milk. Cinnamon EO chemically characterized by gas chromatography/mass spectrometry (GC-MS). Minimum Inhibitory Concentration (MIC) Bactericidal (MBC) were determined, as wel...