Abstract Background and Aims Hypoxia-inducible factor prolyl hydroxylase inhibitor (HIF-PHI) activates HIF in renal pericytes fibroblasts to promote erythropoietin production erythropoiesis. However, the effects of stabilization erythroid lineage are not clear. We aim study lineage-specific on erythropoiesis by preclinical models. Method EporGFP-Cre/+;VhlF/F mice were used achieve HIF. Surface ...

Background: Mesenchymal stem cells (MSCs) are beneficial for cell and gene therapy approaches. These highly potent in producing various cells, including adipocytes, osteoblasts, hematopoietic etc., particularly bone marrow-derived. High proliferation regenerative capacity important phenotypes of MSCs which contribute to the recovery process at damaged sites. Various techniques have been used, i...

introduction: cyclosporin a (cya) is a potent immunosuppressive drug widely used in transplant settings due to its specific inhibition of t–cell activation. several studies have reported the synergistic effect of cya with hematopoietic growth factor in colony culture of mice bone marrow. we studied the effect of il-3, il-6, scf,cya and nutrition medium ( condition medium - pha) on the developme...

Objective: Ionizing radiation produces free radicals which induce DNA damage and cell death. Origanum vulgare leaf extract (OVLE) is a natural compound and its capability of scavenging free radicals and its antioxidant activity have been demonstrated by many researchers. In this study, using micronucleus assay, radioprotective effect of OVLE against clastogenic and cytotoxic effect of gamma irr...

Objective(s):To study the effect of low-frequency vibration on bone marrow stromal cell differentiation and potential bone repair in vivo. Materials and Methods:Forty New Zealand rabbits were randomly divided into five groups with eight rabbits in each group. For each group, bone defects were generated in the left humerus of four rabbits, and in the right humerus of the other four rabbits. To t...

Objective- To investigate and compare growth potential as well as aging of mesenchymal stem cells (MSCs) derived from rat bone marrow tissue and adipose tissue (AT) occurred at epicardial and epididymal regions. Design- Experimental study.   Animals- 10 Wistar Rats.   Procedures- Rat MSCs occurred at bone marrow and epicardial and epididymal AT were isolated and culture expanded through sev...

Objectives- To isolate, culture-expand and differentiate mesenchymal stem cells from ovine bone marrow and determine their culture requirements for high expansion rate. Design- Experimental study. Animals- Five Shal sheep. Procedures- In this study, ovine marrow cells were plated and culture expanded through 3 successive subcultures. The resultant cells were then plated at differentiating condi...

Background: Finding a suitable method for rapid, accurate and reliable estimation of absorbed dose has high priority in management of the radiation exposed persons. Shortly after radiation exposure, apoptosis is a major detriment in proliferative tissues such as the hematopoietic system. Therefore, quantification of apoptosis in these tissues could be useful for rapid estimation of radiation ex...

Background: Type 2 diabetes (T2D) in the elderly is an epidemic that has major impact on global health. Thus this experimental study aimed to compare the response of microRNA-133a (miR-133a) in different tissues and of Runt-related transcription factor 2 (Runx2) in bone marrow tissue following resistance and endurance training in old rats with high-fat diet and streptozotocin (HFD/STZ)-induced ...

high-dose chemotherapy and autologous stem cell transplantation (sct) have become an effective care for many patients with hematological malignancies. harvesting the stem cells is one the most important parts of sct. the two most commonly used mobilization regimens are the use of granulocyte colony-stimulating factor (g-csf) or g-csf plus chemotherapy. however, about 10-30% of patients are unab...