The ae (amylose extender) recessive mutant alleles in maize are an important genetic resource for the development of high-amylose cultivars. On the basis of ae allele sequences (from the National Center for Biotechnology Information), the ae mutant alleles were cloned from high-amylose maize and the allelic Ae gene from common maize luyuan92 inbred lines. Five pairs of primers were designed to ...

Expression of the maize alcohol dehydrogenase 1 (Adh1) gene is transcriptionally regulated under conditions of anaerobic stress. DNA sequences required for the expression of Adh1 have been identified by a functional analysis of in vitro constructed hybrid genes consisting of the Adh1 promoter fused to the chloramphenicol acetyltransferase coding region. A series of 5' deletions, 3' deletions, h...

A comparison of oomycete diversity in reed stands ( Phragmites australis ) of Lake Constance, Germany, and maize fields close by provided evidence for the occurrence of natural hybridization between Pythium phragmitis , a newly described reed pathogen, and an as-yet unknown Pythium species closely related to P. phragmitis and P. arrhenomanes . Internal transcribed spacer and β -tubulin sequence...

Clavibacter michiganensis subsp. nebraskensis (Cmn), the causal organism of Goss's wilt and leaf blight of maize, can be detected in the phyllosphere of its host prior to disease development. We compared the morphology and pathogenicity of 37 putative isolates of Cmn recovered from asymptomatic and symptomatic maize leaves. Thirty-three of the isolates produced mucoid orange colonies, irrespect...

water deficit and low nitrogen content of soil are the two major yield limiting factors in most maize (zea mays l.) producing areas. the objective of the current research was to evaluate the relationships between photosynthetic water and nitrogen use efficiency at different levels of soil moisture and nitrogen contents. a factorial experiment, based on completely randomized design with 3 replic...

since crosses of lines with suitable genetic distance produce excellent hybrids, the identification of genetic diversity between zea mays l. inbred lines in hybrid maize breeding programs has a high priority. a total of 27 inbred lines plus b73 and mo17 (sc704 parents) were evaluated by 15 inter-simple sequence repeat (issr) primers. inbreds were selected randomly from inbred lines from the agr...

this study was conducted to investigate the effects of applying deficit irrigation on aboveground biomass, leaf area and root biomass for maize hybrid 704 single-cross. experimental treatments included 4 different irrigation levels, 5 growth stages with three replicates in a completely randomized factorial statistical design. aboveground biomass, leaf area and root biomass in 4 longitudinal sec...

As an extremely important staple food throughout East and Southern Africa, maize (Zea mays) has been a primary focus of agricultural research on the continent during and since the Green Revolution. Despite the development of higher yielding varieties (HYVs) and improved cultivation methods, Kenya has yet to attain self-sufficiency in the crop's production and is a regular importer of grain and ...

Maize is an important staple food in Nigeria. Declining yields of maize as a result of several environmental and biological factors have necessitated technological innovations focusing on maturity time, disease resistance and palatability of the crop. This study therefore assesses the adoption of improved seeds and efficiency levels of farmers in River state, Nigeria. The data were obtained fro...

Lysine is the most limiting essential amino acid for animal nutrition in maize grains. Expression of naturally lysine-rich protein genes can increase the lysine and protein contents in maize seeds. AtMAP18 from Arabidopsis thaliana encoding a microtubule-associated protein with high-lysine content was introduced into the maize genome with the seed-specific promoter F128. The protein and lysine ...