The human cytomegalovirus (HCMV) gene products US2 and US11 dislocate major histocompatibility class I heavy chains from the ER and target them for proteasomal degradation in the cytosol. The dislocation reaction is inhibited by agents that affect intracellular redox potential and/or free thiol status, such as diamide and N-ethylmaleimide. Subcellular fractionation experiments indicate that thi...

Two novel pendant-armed dialdehydes (1a and 1b) were prepared by a one-step reaction between 5-chloro-3-(chloromethyl)-2-hydroxybenzaldehyde/5-methyl-3-(chloromethyl)-2-hydroxybenzaldehyde and cyclohexylamine involving two nucleophilic substitutions, and they were used to react with 1,3-propanediamine to prepare Schiff-base macrocyclic complexes in the presence of ZnX2 salts (X = Cl, Br, and I)...

A practical synthesis of a tetrahydroaminoquinoline scaffold (12) was developed that used a stereocontrolled aza Michael as the key reaction. Three tetrahydroquinoline alkaloid-like, tricyclic derivatives 16, 18, and 19 with different medium to macrocyclic ring skeletons were obtained, using this scaffold as the starting material, in a modular manner. The macrocyclic compounds with an isolated ...

in this paper, cubic nickel oxide nanoparticles were successfully prepared by solid-state thermal decomposition of nickel(ii) macrocyclic schiff-base complex at 450°c for 3 h without employing toxic solvent or surfactant and complicated equipment. nickel(ii) macrocyclic schiff-base complex was synthesized by the reaction of 1,2-bis(2-formyl-3-methoxyphenyl)propane, nicl2•6h2o and 1,3-phenylened...

Chick embryo corneas (stages 38 and 45) have been used to study variations in pentose shunt activity following the use of a glutathione-specific oxidizing agent, diamide, and a sulfydryl blocking agent, N-ethylmaleimide (NEM). Shunt activity was measured by the ratio of radiolabeled carbon 1 (14C-1) of glucose to radiolabeled carbon 6 (14C-6) of glucose derived as expired 14CO2. Diamide and NEM...

The influence of oxidation of membrane thiol groups on lysosomal proton permeability was studied by measuring lysosomal pH with FITC-conjugated dextran, determining the membrane potential with 3,3'-dipropylthiadicarbocyanine iodide and monitoring their proton leakage with p-nitrophenol. Residual membrane thiol groups were measured with 5,5'-dithiobis-(2-nitrobenzoic acid). The lysosomal membran...

The asymmetric unit of the title cocrystal, C(14)H(14)N(4)O(2)·C(14)H(10)O(4)S(2), comprises a twisted 2,2'-(disulfanediyl)dibenzoic acid mol-ecule [dihedral angle between the benzene rings = 76.35 (10)°] and a U-shaped N,N'-bis-(3-pyridyl-meth-yl)ethane-diamide mol-ecule with the pyridyl groups lying to the same side of the central diamide moiety [C-C-C-N = 113.8 (2) and -117.6 (2)°]. The latt...

A common perturbation found in cells under oxidative stress is alteration in cellular Ca2+ homeostasis. In order to understand the effects of such oxidative damage, human red cell plasma membrane Ca(2+)-ATPase (PMCA) was studied by measuring PMCA activity, both in the presence and absence of calmodulin (CaM), following treatment with sulfhydryl agents, N-ethylmaleimide, iodoacetate and diamide....