BACKGROUND Expression of autonomous bioluminescence from human cells was previously reported to be impossible, suggesting that all bioluminescent-based mammalian reporter systems must therefore require application of a potentially influential chemical substrate. While this was disproven when the bacterial luciferase (lux) cassette was demonstrated to function in a human cell, its expression req...

Listeria monocytogenes is a Gram-positive facultative intracellular pathogen that is widely used as a model organism for the analysis of infection biology. In this context, there is a current need to develop improved reporters for enhanced bioluminescence imaging (BLI) of the pathogen in infection models. We have developed a click beetle red luciferase (CBR-luc) based vector (pPL2CBRopt) expres...

The cold-water-fish pathogen Vibrio salmonicida expresses a functional bacterial luciferase but produces insufficient levels of its aliphatic-aldehyde substrate to be detectably luminous in culture. Our goals were to (i) better explain this cryptic bioluminescence phenotype through molecular characterization of the lux operon and (ii) test whether the bioluminescence gene cluster is associated ...

M e t h o d s Data sources: MEDLINE (1975 to July 2003), the Cochrane Library, and bibliographies of relevant reviews and studies. Study selection and assessment: Englishlanguage randomized controlled trials (RCTs) of adults 18 to 65 years of age with a diagnosis of mood disorder based on DSMIII, DSM-III-R, DSM-IV, Research Diagnostic Criteria, or the Rosenthal criteria, who were in the acute p...


 In general, oil palm plants are cultivated using a monoculture system. The system is profitable from an economic perspective and harms the environment; one microclimate. One of efforts to reduce negative impact apply agroforestry with agarwood. This research conducted on smallholder plantations in Bukit Kemuning Village, Tapung Hulu Sub District, Kampar Riau Province. was survey method, ...

We have measured the illuminance (brightness) of seven neonatal units during both the day and the night. When the units were lit solely by fluorescent tubes the mean illuminance was 348 lux (range 192-690). During the day the mean illuminance was 470 lux (range 236-905). The high dependency regions in four of the seven units were significantly brighter than the corresponding low dependency nurs...

A mathematical model of the Lux luminescence system, governed by the operon luxCDABE in the terrestrial bacterium Photorhabdus luminescens, was constructed using a set of coupled ordinary differential equations. This model will have value in the interpretation of Lux data when used as a reporter in time-course gene expression experiments. The system was tested on time series and stationary data...

This study was conducted to investigate the effect of the exposure to dim light during sleep on the brain activation while conducting tasks requiring working memory. 23 young healthy subjects participated in this study. The subjects were instructed to sleep in a polysomnography room with no light exposure on the first night and under a dim light condition of 5 lux or 10 lux on the second night....

OBJECTIVE Concern that disturbances of sleep and light exposures at night might increase cancer risks have been expressed, but little actual exposure data has been collected. Measurements from a representative population sample were examined to understand the magnitude of in-bed light exposure at night and possible correlates. METHODS From 1990 to 1994, a home survey of sleep disorders among ...

Bioluminescence generated by the Vibrio fischeri Lux system consumes oxygen and reducing power, and it has been proposed that cells use this to counteract either oxidative stress or the accumulation of excess reductant. These models predict that lux expression should respond to redox conditions; yet no redox-responsive regulator of lux is known. We found that the luxICDABEG operon responsible f...