Today, 2D online or offline liquid chromatography/mass spectrometry is state of the art for the identification of proteins from complex proteome samples in many laboratories. Both 2D liquid chromatography methods use two orthogonal liquid chromatography separation techniques. The most commonly used techniques are strong cation exchange chromatography for the first dimension and reversed phase s...

One of the modern techniques of mixture separation is the application of liquid membranes. They reveal the ability of selective transport of mixture components in which a liquid membrane constitutes a separate phase which separates two other liquid or gas phases. This property of membranes makes them useful in the textile and food industries, in hydrometallurgy, medicine, biotechnology, environ...

We have chemically crosslinked a globular protein, gamma IIIb-crystallin, to produce a system of well-defined oligomers: monomers, dimers, trimers and a mixture of higher n-mers. Gel electrophoresis, size exclusion chromatography, quasielastic light scattering spectroscopy, and electrospray ionization mass spectrometry were used to characterize the oligomers formed. The liquid-liquid phase sepa...

In the past, investigations of phase separation in concentrated polymer solutions centered first of all on the crystallization kinetics (1) and morphology (2) of polymer crystals growing from solutions and, secondly, on the equilibrium thermodynamic properties of liquid-liquid phase separation phenomena (3). Papkov and Yefimova (4) have recently given a classification of different types of phas...