The activity of Chromobacterium viscosum lipase for hydrolysis of olive oil in sodium bis(2-ethylhexyl) sulfosuccinate (AOT) reverse micelles was increased by pretreatment with acetone. In contrast to the untreated lipase, no sharp fall in the activity of the treated lipase at higher W0 (water to AOT molar ratio) values was observed. The fluorescence emission intensity of the treated lipase in ...

The liver contains an acy~glycerol hydro~ase activity (liver-lipase) which is releasable by heparin perfusion [ 11. Recent work has indicated that this lipase may be involved in the cellular uptake of high density lipoprotein p~~osp~lolip~ds and cholesterol from the plasma [2,3]. It has been suggested that high density Iipoprotein phospllolip~ds can be hydrolyzed by the enzyme, thereby enabling...

To avoid the secondary pollution that inorganic corrosion inhibitor may cause in circulating cooling water, the bio-corrosion inhibition effect of lysozyme, catalase, lipase, and laccase, as the biologic inhibitors without the above problem, and the increased bio-corrosion inhibition effect of Ca+2 and Mg+2 are studied. An enzyme with corrosion properties was selected by rotary coupon test. The...

the present study was aimed to isolate and characterize the lipolytic enzyme producing bacteria from soil samples of regions around zayande-rood river of isfahan, iran. soil samples were collected from 15 cm depth of soil surface. based on morphology, distinct colonies were isolated and purified through streak culture on to standard agar plates. isolated colonies were examined for lipase activi...

BACKGROUND Pichia guilliermondii was found capable of expressing the recombinant thermostable lipase without methanol under the control of methanol dependent alcohol oxidase 1 promoter (AOXp 1). In this study, statistical approaches were employed for the screening and optimisation of physical conditions for T1 lipase production in P. guilliermondii. RESULT The screening of six physical condit...

Lipoprotein lipase activity was higher in fat-pad pieces than in isolated adipocytes from the same fed rats, whereas hydrolysis of triacylglycerols from triacylglycerol-rich lipoproteins was similar in the two preparations when incubated either in basal conditions or in the presence of heparin. In both preparations there was a similar release of lipoprotein lipase activity into the medium durin...

Background: Lipase is an enzyme with immense application potential. Ester synthesis by lipase catalysis in organic media is an area of key industrial relevance. Enzymatic preparations with traits that cater to the needs of this function are hence being intensely researched. Objective: The objectives of the study were to immobilize the lipase from Bacillus sp. PS35 by cross-linking and adsorpti...

A gene encoding a thermotolerant lipase with broad pH was isolated from an Antarctic Pseudomonas strain AMS3. The recombinant lipase AMS3 was purified by single-step purification using affinity chromatography, yielding a purification fold of approximately 1.52 and a recovery of 50%. The molecular weight was approximately ∼60 kDa including the strep and affinity tags. Interestingly, the purified...

Heretofore most methods for the determination of lipase activity have employed emulsions of fatty acid esters in water (1). These emulsions have been stabilized by use of such agents as gum acacia. The disadvantages of such methods are that (1) the emulsions break up to a considerable and variable extent during the course of the incubation, and (2) the enzyme is presumed to be in the aqueous ph...

The molecular defects resulting in a deficiency of lipoprotein lipase activity in a patient with the familial hyperchylomicronemia syndrome have been identified. Increased lipoprotein lipase mass but undetectable lipoprotein lipase activity in the patient's post-heparin plasma indicate the presence of an inactive enzyme. No major gene rearrangements were identified by Southern blot analysis of ...