Genome sequencing of cellulolytic myxobacterium Sorangium cellulosum reveals many open-reading frames (ORFs) encoding various degradation enzymes with low sequence similarity to those reported, but none of them has been characterized. In this paper, a predicted lipase gene (lipA) was cloned from S. cellulosum strain So0157-2 and characterized. lipA is 981-bp in size, encoding a polypeptide of 3...

We analyzed the molecular defects in the lipoprotein lipase gene of a patient with type I hyperlipidemia suffering from recurrent pancreatitis, indicative for lipoprotein lipase deficiency. Postheparin lipoprotein lipase activity in the patient was decreased by 70%. Direct genomic sequencing revealed compound heterozygosity for two mutation: the well-known Gly188-->Glu and a new Val69-->Leu sub...

Molecular Modeling is essential tool in the drug design process describes the generation, manipulation or representation of 3D structures of the molecules and associated physico-chemical properties while docking predicts the preferred orientation of one molecule to a second when bound to each other to form a stable complex. A cold active lipase producing potential psychrophilic bacteria (GN) wa...

lipases are diversified enzymes in their properties and substrate specificity, which make them attractive tools for various industrial applications. in this study, an alkaline thermostable lipase producing bacteria were isolated from soil of different regions of isfahan province (iran) and its lipase was purified by ammonium sulfate precipitation and ion exchange chromatography. to select a the...

We have investigated the gene and protein expression of adipose triglyceride lipase (ATGL) and triglyceride (TG) lipase activity from subcutaneous and visceral adipose tissue of lean and obese subjects. Visceral and subcutaneous adipose tissue was obtained from 16 age-matched lean and obese subjects during abdominal surgery. Tissues were analyzed for mRNA expression of lipolytic enzymes by real...

A partial (634 bp) cDNA clone, AF1229, obtained from expressed sequence tags (ESTs) of solid-cultured basidiomes of Antrodia cinnamomea is homologous to the lipase gene in Rhizomucor miehei. 5'-rapid amplification of cDNA ends (RACE) and 3'-RACE amplification showed that the full-length lipase gene, Ac-LIP, has a 912bp open reading frame (ORF), a 183bp 5' non-coding region, and a 144bp 3' non-c...

A severe hyperlipemia in mink, with a pattern that suggested recessive inheritance, was observed at a farm in Norway. On a normal mink diet, affected animals had grossly elevated levels of plasma triglycerides which decreased towards normal on a low-fat diet. Normal minks had the main part of their plasma cholesterol in the HDL fraction. Affected minks, although severely hypertriglyceridaemic, ...

background and objectives: lipases are valuable biocatalysts which are widely used in the detergent, food, dairy and pharmaceutical industries. the aims of the present study included the isolation of a lipase-producer from industrial zones and the partial characterization of the enzyme. materials and methods: a number of bacteria were isolated from sites related to the oil industries. an isolat...

DsbA and DsbC proteins involved in the periplasmic formation of disulfide bonds in Pseudomonas aeruginosa were identified and shown to play an important role for the formation of extracellular enzymes. Mutants deficient in either dsbA or dsbC or both genes were constructed, and extracellular elastase, alkaline phosphatase, and lipase activities were determined. The dsbA mutant no longer produce...

Mutated mouse lipoprotein lipase (LPL) containing a leucine (L) to histidine (H) substitution at position 452 was transferred into mouse liver by hydrodynamics-based gene delivery (HD). Mutated-LPL (MLPL) gene transfer significantly increased the concentrations of plasma MLPL and triglyceride (TG) but significantly decreased the activity of plasma LPL. Moreover, the gene transfer caused adiposi...