This study aimed to evaluate the effect of Wogonoside (Wog), a flavonoid monomer, on hyperlipidemia and explore its possible mechanisms. APOE -/- mice were used establish animal model by feeding high-fat diet (HFD). The serum level triglycerides (TG), total cholesterol (TC), high-density lipoprotein (HDL-c), low-density (LDL-c), inflammatory cytokines measured enzyme-linked immunosorbent assay ...

Modification with gold black considerably increased the detection current of protruding micropillar electrodes in microfluidic electrochemical enzyme-linked immunosorbent assay (ELISA).

An indirect enzyme-linked immunosorbent assay (ELISA) was developed for the detection of IgG serum antibodies to swine Sendai virus. To evaluate the preliminary potential of ELISA, sera from 10 healthy pigs and 34 sera from finishing pigs suffering from respiratory signs were tested. The results were compared with those obtained from the same sera by hemagglutination inhibition assay.

A proficiency testing panel for detecting antibodies against Brucella abortus was developed and evaluated by both primary binding and conventional serological tests, using the guidelines of the World Organisation for Animal Health and the International Organization for Standardization Guide 43-1. All serological tests were judged satisfactory. Among the primary binding tests, the competitive en...

A highly sensitive immuno-PCR method specific for the detection of antigens of and antibodies to bovine herpesvirus 1 was established. This assay attained a high sensitivity of up to 10(7.0) times higher than that of enzyme-linked immunosorbent assay (ELISA) or PCR for antigen detection and 10(5.0) times higher than that of ELISA for antibody detection.

As the gold standard of protein detection, enzyme-linked immunosorbent assay (ELISA) is widely used in medical treatment and biology. Here, we report a digital ELISA method that combines fluorescence-coded magnetic beads with micropore arrays to effectively improve accuracy detection. Fluorescence coded were as solid support ELISA, which modified specifically capture IL-6 serum, then combined g...

Due to the lack of national analogies a prototype enzyme-linked immunosorbent assay (ELISA) test system was developed for qualitative determination antibodies class G hepatitis E virus (HEV) based on using antigenic polypeptides ORF2 and ORF3 3rd genotype (FSBSI «I.I. Mechnikov Research Institute Vaccines Serums», Russia). The results experimental ELISA conducted in parallel with reference «DS-...