LEVEL: INTERMEDIATE R esidual host cell DNA removal in the processing of biological pharmaceuticals is an important metric for consistency of the purification process. There are also safety concerns with respect to the oncogenic potential of residual DNA from continuous cell lines. The acceptable residual amount of DNA is 10 ng/dose for parentally administrated drugs produced from continuous ce...

Manuscript received July 1, 1999. Manuscript revised October 4, 1999. † The authors are with Electromagnetic Compatibility Research Laboratories Co., Ltd., Sendai-shi, 989-3204 Japan. SUMMARY This paper describes a method for estimating current and voltage distributions by scanning with a probe. The method takes advantage of the phenomenon that the coupling between the current and the probe var...

Applied Biosystems has designed and manufactured over 40,000 real-time PCR assays for measuring the expression of genes in humans, mice, and rats. TaqMan Gene Expression Assays each consist of amplification primers (forward and reverse) and a fluorescent labeled TaqMan probe, formulated into a single tube. One of the major user concerns regarding any real-time PCR-based assay product (primers a...

magnetic force microscope ( mfm ) is a powerful technique for mapping the magnetic force gradient above the sample surface. herein, single-wall carbon nanotubes (swcnt) were used to fabricate mfm probe by dielectrophoresis method which is a reproducible and cost-effective technique. the effect of induced voltage on the deposition manner of carbon nanotubes (cnt) on the atomic force microscope (...

Three molecular assays were evaluated for the direct detection of Mycobacterium tuberculosis complex bacteria in 125 respiratory and 22 nonrespiratory samples. The overall sensitivities obtained were as follows: GenoType MTBDRplus, 97.9%; GenoType Mycobacteria Direct, 93.7%; Gen-Probe Mycobacterium tuberculosis Amplified Direct Test, 89.6%. The specificity of the assays used was 100%.

Probe specificity plays a central role in designing accurate microarray hybridization assays. Current literature on specific probe design studies algorithmic approaches and their relationship with hybridization thermodynamics. In this work we address probe specificity properties under a stochastic model assumption and compare the results to actual behavior in genomic data. We develop efficient ...