tan res

BACKGROUND Employing genomic DNA clones to characterise gene attributes has several advantages over the use of cDNA clones, including the presence of native transcription and translation regulatory sequences as well as a representation of the complete repertoire of potential splice variants encoded by the gene. However, working with genomic DNA clones has traditionally been tedious due to their...

In this paper, we investigate the existence of positive solutions for the ellipticequation $Delta^{2},u+c(x)u = lambda f(u)$ on a bounded smooth domain $Omega$ of $R^{n}$, $ngeq2$, with Navier boundary conditions. We show that there exists an extremal parameter$lambda^{ast}>0$ such that for $lambda< lambda^{ast}$, the above problem has a regular solution butfor $lambda> lambda^{ast}$, the probl...

Groups of newborn human infants (N = 180) were habituated to large 16 degrees achromatic ("white") lights of varying luminance (0.35 to 1.16 log cd/m2) and then tested for recovery of habituation to 16 degrees green (dominant lambda = 545 nm), yellow (dominant lambda = 585 nm) or red (dominant lambda = 650 nm) lights which varied in the level of excitation purity (range = 32 to 83%). Results sh...

Genomic analyses of fungal genome structure have revealed the presence of physically-linked groups of genes, termed gene clusters, where collective functionality of encoded gene products serves a common biosynthetic purpose. In multiple fungal pathogens of humans and plants gene clusters have been shown to encode pathways for biosynthesis of secondary metabolites including metabolites required ...

Iron is an important nutrient for the survival and growth of many organisms. In order to survive, iron uptake from the environment must be strictly regulated and maintained to avoid iron toxicity. The ferric uptake regulator protein (Fur) regulates genes involved in iron homeostasis in many bacteria, including phytopathogens. However, to date, the role played by Fur in the biology of Pectobacte...

BACKGROUND Fluorescent reporters are useful for assaying gene expression in living cells and for identifying and isolating pure cell populations from heterogeneous cultures, including embryonic stem (ES) cells. Multiple fluorophores and genetic selection markers exist; however, a system for creating reporter constructs that preserve the regulatory sequences near a gene's native ATG start site h...

Many group I introns encode endonucleases that promote intron homing by initiating a double-strand break-mediated homologous recombination event. A td intron-phage lambda model system was developed to analyze exon homology effects on intron homing and determine the role of the lambda 5'-3' exonuclease complex (Red alpha beta) in the repair event. Efficient intron homing depended on exon lengths...

Inthis paper, the main aim is to introduce the class $mathcal{U}_p(lambda,alpha,beta,k_0)$ of $p$-harmonic mappings togetherwith its subclasses $mathcal{U}_p(lambda,alpha,beta,k_0)capmathcal {T}_p$ and $mathcal{U}_p(lambda,alpha,beta,k_0)capmathcal {T}_p^0$, andinvestigate the properties of the mappings in these classes. First,we give a sufficient condition for mappings to be in $mathcal{U}_p(l...

The baculovirus expression system is one of the most successful and widely used eukaryotic protein expression methods. This short review will summarise the role of bacterial artificial chromosomes (BACS) as an enabling technology for the modification of the virus genome. For many years baculovirus genomes have been maintained in E. coli as bacterial artificial chromosomes, and foreign genes hav...