As a member of phenoloxidase group of enzymes, laccase has been reported as to be quite efficient in oxidative degradation of various phenolics by some investigators. In this paper, effectivity of laccase enzyme produced from Trametes versicolor ATCC 200801 on dechlorination of pp’DDT in order to reduce its toxicity has been investigated. In experimental conditions used during enzymatic dechlor...

Laccases (benzenediol: oxygen oxidoreductase, EC 1.10.3.2) are the extracellular most extensively studied group of enzymes that catalyze the oxidation of a broad range of aromatic amines and phenolic compounds. Among the various fungi, white rot fungi are the well-known and potent producers of Laccase [1]. In recent years Laccase have attracted commercial significance due to their intensive use...

Volvariella volvacea, strain V14, produces multiple forms of extracellular laccase when grown in submerged culture in a defined medium with glucose as sole carbon source, and on cotton waste 'compost' representative of the conditions used for industrial-scale mushroom cultivation. In liquid culture, enzyme synthesis is associated with the onset of secondary growth, and is positively regulated b...

Regulation of alkaline-resistant laccase from Perenniporia tephropora KU-Alk4 was proved to be controlled by several factors. One important factor was the initial pH, which drove the fungus to produce different kinds of ligninolytic enzymes. P. tephropora KU-Alk4 could grow at pH 4.5, 7.0, and 8.0. The fungus produced laccase and MnP at pH 7.0, but only laccase at pH 8.0. The specific activity ...

Microdroplets in water-in-oil emulsions can be used as microreactors with volumes 10(3) to 10(9) times smaller than the smallest working volumes in a microtitre plate well (1-2 microL). However, many reactions and assays require multiple steps where new reagents are added at defined times, to start, modify or terminate a reaction. The most flexible way to add new reagents to pre-formed droplets...

Endocrine disrupting chemicals (EDC’s) are industrial and pharmaceutical pollutants, however some EDC’s (estrogens) are naturally synthesized by humans and animals. EDCs can reach waterways through wastewater facility effluent since many treatment plants are not yet capable of removing these pollutants. Research has shown that white rot fungi have the ability to break down wastewater contaminan...

We report in this study that the sonication of laccase from Trametes villosa and bovine serum albumin promotes the formation of protein aggregates with high molecular weight. The formation of aggregates leads to the deactivation of the enzyme, fact that was confirmed by the analysis of the enzyme stability (half-life time) upon ultrasound treatment. This inactivation was mainly caused by the ra...

In order to study the effect of external ethylene on postharvest display and activity of some senescence-related enzymes in potted miniature roses, this experiment was carried out in a completely randomized design, using three treatment levels with three replications. In glass chambers, potted miniature rose plants were exposed to ethylene concentrations of 0, 5 and 10 μL L-1 for 12 h. The resu...

Industrial effluents of textile, paper, and leather industries contain various toxic dyes as one of the waste material. It imparts major impact on human health as well as environment. The white rot fungus Pycnoporus cinnabarinus Laccase is generally used to degrade these toxic dyes. In order to decipher the mechanism of process by which Laccase degrade dyes, it is essential to know its 3D struc...

Production of lignolytic enzymes by the mushroom fungus Stereum ostrea in liquid medium under conditions of vegetative growth was examined for 10 days in comparison to the reference culture Phanerochaete chrysosporium. Though growth and secretion of extracellular protein by S. ostrea were comparable to those of P. chrysosporium, yields of laccase enzyme by S. ostrea were higher than laccase tit...