Very rapid amplification of DNA by PCR in small volumes can be continuously monitored by the detection of the binding of probes with a rapid cycler with built-in fluorometric detection. Primers were designed to amplify approximately 100 bp of the polymerase gene of hepatitis B virus (HBV) spanning codon 550, where mutations associated with resistance to lamivudine invariably occur. Four hybridi...

The c-fos immunohistochemical method of activity-dependent labelling was used to localise locomotor activated neurons in the adult cat spinal cord. In decerebrate cats, treadmill locomotion was evoked by electrical stimulation of the mesencephalic locomotor region (MLR). Spontaneous or MLR-evoked fictive locomotion was produced in decerebrate animals paralysed with a neuromuscular blocking agen...

1. Studies were carried out to test the assumptions involved in the calculation of free fatty acid (FFA) and triglyceride transport rates from the plasma radioactivity curves after the intravenous administration of labelled fatty acid or of pre-labelled lipoprotein triglyceride. 2. Plasma FFA equilibrates reversibly with the interstitial lymph. This makes it necessary to use either a pulse inje...

Mice were given 4 injections of [H]thymidine at 2-hourly intervals and killed at times varying from 1 h to 12 days after the last injection. Autoradiographs of spleen, oesophagus and heart were prepared by the stripping-film technique and exposed for 21, 189 and 367 days. Groups of lymphoid cells surrounding trabeculae were heavily labelled, the decline in grain count suggesting division of the...

desalination plants are potentially capable of having impacts on coastal marine environments due to the production of wastewater with high concentrations of salts (brine discharge) and some heavy metals. the aim of this study was to examine the concentration of accumulated metals (ni, cu, cd, fe, zn, pb) in the sediments near the brine discharge point in the chabahar bay. twenty one sediment sa...

Functional magnetic resonance imaging (fMRI) provides a spatially accurate measure of brain activity. Real-time classification allows the use of fMRI in neurofeedback experiments. With limited labelled data available, a fixed pre-trained classifier may be inaccurate. We propose that streaming fMRI data may be classified using a classifier ensemble which is updated through naive labelling. Naive...

ba ckground: although it is widely accepted that the pre-school age is a critical period  in  the  development of  obesity,  there  are  few  published data  on  factors contributing to variation of total energy expenditure in this age group.to investigate the contribution of body composition, gender and activity on total energy expenditure (tee) in pre-school children. m ethods: the total ener...

since the report from the national high blood pressure (bp) education program working group on bp in children and adolescents and the introduction of a new description called prehypertension many data have been provided on its rate of progression to hypertension, its prevalence and association with other cardiovascular (cv) risk factors and its therapy. making a diagnosis of prehypertension in ...