Isolated hepatocytes prepared from sheep fed a basal diet (bromegrass hay-corn, 50:50 wt/ wt, as-fed basis) with or without urea were used to determine the effects of added ammonia (as NH4Cl) and propionate on the partitioning of C from 1.25 mM L-[1-14C]alanine between oxidation and gluconeogenesis, and the flux of 15N from 1.25 mM L-[15N]alanine to [14N15N]urea and [15N15N]urea. Hepatocyte sus...

Peptides have shown Promising effect as pharmaceutics with the potential to treat a widevariety of diseases. Peptides are mostly synthesized by biological technology or chemicalmethods. Solution phasepeptide synthesis (SPS) and solid phase peptide synthesis (SPPS) aretwo major chemical techniques for peptidesproduction.In this research, the synthesis ofdipeptde(β-alanine-L-histidine)wasexamined...

The identification of reliable biomarkers, such as amino acids, is key for the search extraterrestrial life. A large number microorganisms metabolize, synthesize, take up and excrete acids part acid metabolism during aerobic and/or anaerobic respiration or in fermentation. In this work, we investigated whether microbial could leave a secondary biosignature indicating biological activity environ...

A novel and efficient one-pot three-enzyme cascade method for the synthesis of β-alanine from maleic acid was developed. Two recombinant E. coli strains were constructed. The (MaiA-AspA) co-expressing cis-trans isomerase (MaiA) L-aspartase (AspA) catalyzed biotransformation to L-aspartate via fumaric acid, (ADC) expressing L-aspartate-α-decarboxylase bioconversion β-alanine. After systematic op...

The synthesis of well-defined homo- and co-polypeptides L-alanine (L-Ala) by ring-opening polymerization (ROP) its N-carboxyanhydride (NCAs), either via suspension/interfacial- or solution phase-polymerization using 10-undecene-1-amine ?,?-diamino poly(ethylene glycol) (8 1.5 kDa) as primary amine initiators is reported. When conducting ROP NCA-alanine under suspension/interfacial conditions, b...

Efficiency of the enzymatic metaraminol production with amine donor l -alanine was increased by applying reactive extraction as suitable in situ product removal strategy.