Bilirubin glucuronoside glucuronosyltransferase (EC 2.4.1.95) converts bilirubin monoglucuronide to bilirubin diglucuronide and is concentrated in plasma membrane-enriched fractions of rat liver homogenates. The enzyme was purified 2,000-fold to homogeneity from rat liver. The p1 of the enzyme is 7.9 + 0.2. The enzyme has a molecular weight of 160,000 and is an oligomer of 28,000 dalton subunit...

BACKGROUND Delayed diagnosis of biliary atresia is an important cause of pediatric end-stage liver failure and liver transplantation. We sought to determine whether direct bilirubin is underutilized by retrospectively reviewing patients with biliary atresia. Further, we aimed to determine the role of reflex testing for direct bilirubin in patients suspected for jaundice. METHODS The time inte...

Our aim was to study the usefulness of jaundice visual assessment combined with skin bilirubin determination in 517 healthy newborns. Yellowness assessment was made and babies were included in three different bilirubin classes. Skin bilirubin and total serum bilirubin were determined within 10 minutes from the visual assessment. This latter led to underestimation of serum bilirubin in 16.7-40.4...

One of the causes infant death outside womb is hyperbilirubinemia. This hyperbilirubinemia a clinical phenomenon often found in newborns first week life. In laboratory examination, determination hemoglobin level an indicator that widely used to determine prevalence anemia. study uses observation method with descriptive approach. The data collection this research was carried out at RSIA Pertiwi ...

Bilirubin glucuronoside glucuronosyltransferase (EC 2.4.1.95) converts bilirubin monoglucuronide to bilirubin diglucuronide and is concentrated in plasma membrane-enriched fractions of rat liver homogenates. The enzyme was purified 2,000-fold to homogeneity from rat liver. The pI of the enzyme is 7.9 +/- 0.2. The enzyme has a molecular weight of 160,000 and is an oligomer of 28,000 dalton subun...

The negative interference caused by haemolysis in manual bilirubin assays contrasts with the positive interference reported for some automated methods utilizing the same basic chemistry. A comparison was therefore made of the haemolysis interference associated with several automated bilirubin methodologies: multilayer film (Kodak Ektachem- total bilirubin (TBil), direct bilirubin (DBil), conjug...

The yellow pigments observed in the ocular humors of premature infants were identified as the plasma pigments bilirubin and oxyhemoglobin. The blood/vitreous humor barrier to bilirubin was estimated to be (1/0.11) for total bilirubin and (1/0.25) for direct-acting bilirubin. Hall method histochemical staining for bilirubin in sections of premature infant eyes was highly positive for the vitreou...

infant jaundice is observed during the first week of life in approximately 60% of term and 80% of preterm infants. hyperbilirubinemia may lead to the development of kernicterus, hearing loss, and convulsion. the goal of therapy in hyperbilirubinemia is lowering blood bilirubin levels or at least preventing its increase. it is recommended that phototherapy and if unsuccessful, blood exchange tra...