An assay to identify Cryptosporidium DNA in bovine feces has been developed emphasizing standardization of sample preparation and simplification of the DNA recovery process for PCR amplification and DNA hybridization detection. The Cryptosporidium DNA recovery-PCR detection procedure (CR-PCR) can recover DNA suitable for PCR amplification without using or generating hazardous chemicals or waste...

The efficacy of orally given bicyclomycin on shigellosis was studied in rhesus monkeys infected with Shigella fIexneri 2a. All the animals developed lethal infection after rectal inoculation of the virulent strain 5503. Bicyclomycin and the kanamycin control were given orally at daily dosage of 40 mg;kg for a period of 5 days beginning 24 hours after the inoculation. Bicyclomycin compared favor...

Abstract Livestock waste such as feces, urine, and leftover feed left without further treatment can harm the environment in form of air, water, soil pollution. Therefore, efforts are needed to process livestock into useful products organic fertilizers. that has potential be a source fertilizer is etawa goat waste. Etawa feces processing technology bokashi made from fermented agricultural wastes...

In their first 150 days of lactation, nine Ragusana jennets were investigated at the 'Istituto Sperimentale Zootecnico per la Sicilia' (ISZS) in Palermo, to study the effects of different milking typologies on milk production. The jennets were kept in a paddock and were fed hay ad libitum and concentrate (3.5 kg jennet/day). From post-foaling day 21, every 3 weeks individual milk amounts were r...

Lev, Meir (Albert Einstein College of Medicine, New York, N.Y.), Raymond H. Alexander, and Stanley M. Levenson. Stability of the Lactobacillus population in feces and stomach contents of rats prevented from coprophagy. J. Bacteriol. 92: 13-16. 1966.-Lactobacilli were enumerated in the feces of rats prevented from coprophagy by tail-cupping. No differences were found when numbers of these organi...

Coprophagy occurs in a number of animal species, including nonhuman primates. During the 2003-2004 dry seasons at the Beza Mahafaly Special Reserve, Madagascar, we observed wild ring-tailed lemurs (Lemur catta) consuming dried fecal matter from three different species. Ring-tailed lemurs consumed human feces on 12 occasions, cattle feces twice, and feral dog feces once. Coprophagy in this popul...

A fecal analysis survey was undertaken to quantify animal inputs of pathogenic and indicator microorganisms in the temperate watersheds of Sydney, Australia. The feces from a range of domestic animals and wildlife were analyzed for the indicator bacteria fecal coliforms and Clostridium perfringens spores, the pathogenic protozoa Cryptosporidium and Giardia, and the enteric viruses adenovirus, e...

Using real-time PCR, we tested 15 wolf (Canis lupus) feces from the Superior National Forest (SNF), Minnesota, USA, and 191 from Yellowstone National Park (YNP), USA, collected during summer and 13 during winter for canine parvovirus (CPV)-2 DNA. We also tested 20 dog feces for CPV-2 DNA. The PCR assay was 100% sensitive and specific with a minimum detection threshold of 10(4) 50% tissue cultur...

sodium isotopes were measured i n the pref l ight and pos t f l ight urine and feces, and those feces specimens collected during the manned Apollo 3 Trl missions, by analysis of the urine s a l t s and t h e raw feces i n large crysta3 multidimensional gamma-ray spectrometers. The 7 ~ e was chemically a 3-@I separated, and i t s concentration measured i n an a l l ~a1(1~1), anticoincidence Z sh...

A chemical characteristic of the feces of diarrheal piglets permits differentiation among piglets receiving antibiotic treatment and those with colibacillosis or dyspepsia. A high concentration of lactic or succinic acid was observed in the diarrheic feces of piglets receiving antibiotic treatments and those with dyspepsia; however, no lactic or succinic acids were detected in piglets with coli...