نتایج جستجو برای: its rdna
تعداد نتایج: 1960115 فیلتر نتایج به سال:
We assessed the utility of eight DNA sequence markers (5.8S rDNA, 18S rDNA, 28S rDNA, ITS regions, long-wavelength opsin, elongation factor 1-alpha, cytochrome b, and cytochrome oxidase I) in reconstructing phylogenetic relationships at various levels of divergence in gallwasps (Hymenoptera: Cynipidae), using a set of eight exemplar taxa. We report sequence divergence values and saturation leve...
این زیر جنس که اغلب از اندام های هوایی غلات در مناطق شمال و شمال غرب و تعدادی هم از سایر بسترها در ایران به دست آمده بودند، مورد مطالعه قرار گرفتند. براساس داده های ریخت شناسی و مولکولی (توالی های مناطق ژنی its rdna و β-tubulin)، تعداد نه گونه aspergillus متعلق به زیرجنس nidulantes شناسایی گردید که در بخش های nidulantes، usti، terrei و flavipedes گروه بندی شدند. آنالیزهای فیلوژنتیکی انجام گرفته...
سفیدک پودری از بیماریهای مهم گوجهفرنگی در مزارع و گلخانههای دنیا محسوب میشود و در ایران گونه leveillula tauricaبهعنوان تنها عامل این بیماری گزارش شده است. طی مطالعه قارچهای تیره erysiphaceae در استان اردبیل، برگهای گوجهفرنگی با علایم سفیدک پودری از گلخانهای در شهر اردبیل جمعآوری و جدایه ها بر اساس ویژگی های ریخت شناختی مورد شناسایی اولیه قرار گرفتند. سپس dna ژنومی قارچ به روش chelex 5...
RNA polymerase I-catalyzed synthesis of the Schizosaccharomyces pombe approximately 37S pre-rRNAs was shown to be sensitive to regulatory sequences located several kilobases upstream of the initiation site for the rRNA gene. An in vitro transcription system for RNA polymerase I-catalyzed RNA synthesis was established that supports correct and activated transcription from templates bearing a ful...
* At present, molecular ecological studies of arbuscular mycorrhizal fungi (AMF) are only possible above species level when targeting entire communities. To improve molecular species characterization and to allow species level community analyses in the field, a set of newly designed AMF specific PCR primers was successfully tested. * Nuclear rDNA fragments from diverse phylogenetic AMF lineages...
Arguably more mycophiles hunt true morels (Morchella) during their brief fruiting season each spring in the northern hemisphere than any other wild edible fungus. Concerns about overharvesting by individual collectors and commercial enterprises make it essential that science-based management practices and conservation policies are developed to ensure the sustainability of commercial harvests an...
The 16S rDNA polymerase chain reaction (PCR)-denaturing gradient gel electrophoresis (DGGE) and sequencing method has been demonstrated to be valuable in detecting pathogens in the blood of patients suffering from fever or neutropenia. However, its use in the diagnosis of neonatal late‑onset septicemia (LOS) has not yet been reported. The aim of the present study was to investigate the efficien...
Globodera millefolii and G. artemisiae are interesting because their type localities (Estonia and Russia, respectively) are geographically distant from those of the potato cyst nematodes and other Globodera species that seem to have originated in the Western world, and because the type host for each is a member of Compositae rather than Solanaceae. Sequence data for ITS1, ITS2, and 5.8S ribosom...
Gummy stem blight is a major foliar disease of muskmelon (Cucumis melo L.). In this study, morphological characteristics and rDNA internal transcribed spacer (ITS) sequences were analyzed to identify the causal organism of this disease. Morphological examination of the Jeonbuk isolate revealed that the percentage of monoseptal conidia ranged from 0% to 10%, and the average length × width of the...
The aim of the study was to detect the rDNA sequences and their regions in Histoplasma capsulatum, which could be considered species-specific and used as a molecular method for this diagnosis by the technique of nested polymerase chain reaction (nested PCR), employing specific sequences (primers) for H. capsulatum: 18S rDNA region (HC18), 100 kDa (HC100) and the sequence 5.8 S-ITS rDNA (HC5.8)....
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