We demonstrate a multiplexed loop mediated isothermal amplification (LAMP) assay for infectious disease diagnostics, where the analytical process flow of target pathogens genomic DNA is performed manually by moving magnetic beads through a series of plugs in a capillary. Heat is provided by a water bath and the results are read by the naked eye, enabling applications in low resource settings.

In the present research, free convection heat transfer from isothermal concave and convex body shapes is studied numerically. The body shapes investigated here, are bi-sphere, cylinder, prolate and cylinder with hemispherical ends; besides, they have the same height over width (H/D = 2). A Numerical simulation is implemented to obtain heat transfer and fluid flow from all of the geometries in a...

In this study, the changes in microstructure and mechanical properties during isothermal heat treatment of cast steel before after hot deformation were investigated using medium-carbon with low alloy content. The microstructural characteristics hot-rolled under heat-treatment conditions examined optical microscopy scanning electron conjunction backscatter diffraction. variation was evaluated Ro...

the effect of partial slip and temperature dependent fluid properties on the mhd mixed convection flow from a heated, non-linearly stretching surface in the presence of radiation and non-uniform internal heat generation/absorption is investigated. the velocity of the stretching surface was assumed to vary according to power-law form. thermal transport is analyzed for two types of non-isothermal...

In this paper, a 0.2 C–1.6 Mn–1.5 Si wt. % TRIP-aided cold-rolled steel sheet was fabricated and the optimal heat treatment conditions (Intercritical Annealing “IA” and Bainitic Isothermal Transformation “BIT”) were investigated to maximize the volume fraction and stability of the retained austenite. The effects of temperature on IA (770, 790 and 810 ºC) and BIT (330, 350 and 370 <su...

In resource-limited settings, the lack of decentralized molecular diagnostic testing and sparse access to centralized medical facilities can present a critical barrier to timely diagnosis, treatment, and subsequent control and elimination of infectious diseases. Isothermal nucleic acid amplification methods, including reverse transcription loop-mediated isothermal amplification (RT-LAMP), are w...

Pressure-induced gelatinization of chickpea flour (CF) was studied in combination with subsequent temperature-induced gelatinization. CF slurries (with 1:5 flour-to-water ratio) and CF in powder form were treated with high hydrostatic pressure (HHP), temperature (T), and treatment time (t) at three levels (200, 400, 600 MPa; 10, 25, 50 °C; 5, 15, 25 min). In order to investigate the effect of s...

The coupling of reactions in catalytic membrane reactors provides novel reactor configurations that allow shifting the thermodynamic equilibrium and yields of thermodynamically limited reactions and enhancing significantly the rate of production. An interesting pair to couple is the dehydrogenation of ethylbenzene to styrene and the hydrogenation of nitrobenzene to aniline. Hydrogen produced in...

In this work, the preliminary results of a process integration analysis of a logistical fuels processing plant is presented. A simulation model of a bench scale test bed was developed using a commercial simulator (Pro/II) and used to generate the necessary data for performing a thermal pinch analysis. The analysis shows that considerable savings can be obtained through increased thermal integra...

There is a lack of studies that examine the dynamics of heat-induced shrinkage of organ tissues. Clinical procedures such as radiofrequency ablation, microwave ablation or high-intensity focused ultrasound, use heat to treat diseases such as cancer and cardiac arrhythmia. When heat is applied to tissues, shrinkage occurs due to protein denaturation, dehydration and contraction of collagen at te...