In this paper we describe the use of specific proteinases, surface-specific radioiodination, and antigenic reactivity in conjunction with isoelectric focusing for probing the conformations of different polioviral empty capsid species. Naturally occurring empty capsids (called procapsids) with an isoelectric point of 6.8 were resistant to proteolytic digestion by trypsin or chymotrypsin, as were...

Basic equipment such as incubation and refrigeration systems plays a critical role in nearly all aspects of the traditional biological research laboratory. Their proper functioning is therefore essential to ensure reliable and repeatable experimental results. Despite this fact, in many academic laboratories little attention is paid to validating and monitoring their function, primarily due to t...

The non-aggregating nature of a water-soluble pi-conjugated polythiophene has been characterised by concentration independent thermal denaturing.

A k-partial permutation out of n elements is a k-tuple (p1, p2, . . . , pk) with k distinct elements and pi ∈ [n] = {1, 2, . . . , n}, i = 1, 2, . . . , k. Let (p1, p2 . . . , pn) be a full permutation of size n, where the elements are distinct and pi ∈ [n], i = 1, 2, . . . , n. Then we say the k-partial permutation (p1, p2, . . . , pk) is induced from the full permutation. A universal cycle on...

An amphiphilic platinum(II)-containing diblock copolymer, with pH- and solvent-responsive micellization properties that could induce drastic UV-vis and emission spectral changes via modulation of Pt...Pt and pi-pi stacking interactions, has been demonstrated in both aqueous and organic media.

BRODA et al. studied frog rhodopsin in regard to the electrophoretic nature1). From the migration velocity of quartz particles on which frog rhodopsin and its bleached product had been adsorbed, they estimated the isoelectric points as being 4.47 and 4.57 respectively. Although a few available informations are suggestive of that the isoelectric point of cattle rhodopsin is rather on the less ac...

1. While crystalline egg albumin is highly soluble in water at low temperature at the pH of its isoelectric point, it is coagulated by heating. It has long been known that this coagulation can be prevented by adding either acid or alkali, whereby the protein is ionized. 2. It is shown in this paper that salts with trivalent or tetravalent ions, e.g. LaCl(3) or Na(4)Fe(CN)(6), are also able to p...

Purified Staphylococcus aureus enterotoxins B and C(1), the causative agents of staphylococcal food poisoning, were resolved into several components by scanning isoelectric focusing in sucrose density gradients in the absence and presence of 6 M urea and 5 mM dithiothreitol. The observed heterogeneity persisted in the denaturing medium, which suggests that the primary structure of protein enter...

1. Reported effects of different conditions on the stability of the purified chlorophyll-protein complex have been confirmed. 2. The electrophoretic behavior of the chlorophyll-protein complex prepared from two unrelated species of plants (Aspidistra elatior and Phaseolus vulgaris) has been investigated and shown to be dissimilar. In M/50 acetate buffer at 25 degrees C, the isoelectric point of...