The purified Immunoglobulin G (IgG) is effectively used in passive immunization. There are various methods to isolate the IgG from serum, with its own advantages and disadvantages. In the current study, a comparative efficacy of the affinity chromatography using Protein A—Sepharose and ion exchange chromatography using DEAE Sephadex A50 was done with regard to yield and purity of IgG. Both meth...

Biological small-angle X-ray scattering (BioSAXS) is a powerful technique to determine the solution structure, particle size, shape and surface-to-volume ratio of macromolecules. However, a drawback is that the sample needs to be monodisperse. To ensure this, size-exclusion chromatography (SEC) has been implemented on many BioSAXS beamlines. Here, the integration of ion-exchange chromatography ...

A. Introduction B. Extraction of Lipids from Biological Fluids 1. Gangliosides 2. Prostaglandins and other eicosanoids 3. Purification of total lipid extracts 4. Some miscellaneous extraction procedures C. Adsorption Chromatography for the Isolation of Lipid Classes 1. Lipid class or group separations on silica gel 2. Adsorption chromatography with chemically-bonded phases D. Reversed-Phase App...

9-Aminononanoic acid, 9-aminononanamide, and related compounds derived from soybean oil by reactions, including reductive ozonolysis and reductive amination, were separated on an analytical scale either by gas liquid chromatography of trifluoroacetylated or trimethylsilylated derivatives or by thin layer chromatography and on a preparative scale by ion-exchange chromatography. Comparative analy...