Background: In Algeria the date wastes production is estimated at least 85.000 tones. Date wastes is an economic source of carbohydrates for conversion to industrial enzymes because it is readily available and relatively low priced. Objective: The aim of the present study was to investigate the potential of using date wastes as a substrate for the production of a-amylase and invertase. Materia...

DNA-protein conjugates are very useful in analytical chemistry for target recognition and signal amplification. While a number of methods for conjugating DNA with proteins are known, methods for purification of DNA-protein conjugates from reaction mixture containing unreacted proteins are much less investigated. In this work, a simple and efficient approach to purify DNA-invertase conjugates fr...

About 25 yo of the total cellular invertase and trehalase activity were found in a purified Neurospora wall preparation. Attempts were made to dissociate these enzymes from the walls with chemical reagents and hydrolytic enzymes. A detergent (Triton X-IOO), a sulphydryl reducing agent (P-mercaptoethanol), a chelating agent (ethylenediaminetetraacetate), a concentrated salt solution (I M-KCl), a...

Sucrose (Suc):Suc 1-fructosyltransferase (1-SST) is the key enzyme in plant fructan biosynthesis, since it catalyzes de novo fructan synthesis from Suc. We have cloned 1-SST from onion (Allium cepa) by screening a cDNA library using acid invertase from tulip (Tulipa gesneriana) as a probe. Expression assays in tobacco (Nicotiana plumbaginifolia) protoplasts showed the formation of 1-kestose fro...

In isolated rat hepatocytes electroloaded with [14C]sucrose, autophaged sugar accumulated in lysosomes under control conditions, and in prelysosomal autophagic vacuoles (amphisomes) in the presence of asparagine, an inhibitor of autophagic-lysosomal fusion. Endocytic uptake of the sucrose-cleaving enzyme invertase resulted in rapid and complete degradation of autophaged sucrose in both amphisom...

The soluble invertase activty in etiolated Avena seedlngs was highest at the apex of the coleoptile and much lower in the primary leaf, mesocotyl, and root. The activity in all parts of the seeding consisted of two invertases (I and II) which were separated by chromatography on diethylaminoethylcellulose. Both enzymes appeared to be acid invertases, but they dHffered in molecular size, pH optim...

Sucrose hydrolysis reaction, catalysed by invertase under microwave irradiation was carried out in aqueous, isopropanol + water and polyethylene glycol + water system. The effect of conventional and microwave heating on the rate of hydrolysis was studied under a range of physical exposure conditions. The catalytic activity of invertase in aqueous and organic solvents were measured at different ...