نتایج جستجو برای: inverse pcr

تعداد نتایج: 264411  

2012
Dong Liu Guoli Zhu Wenqiao Tang Jinquan Yang Hongyi Guo

Short interspersed nucleotide elements (SINEs), a type of retrotransposon, are widely distributed in various genomes with multiple copies arranged in different orientations, and cause changes to genes and genomes during evolutionary history. This can provide the basis for determining genome diversity, genetic variation and molecular phylogeny, etc. SINE DNA is transcribed into RNA by polymerase...

2009
Stephanie Cara Bishop Mark L. Richter David O. Davido

Journal: :BioTechniques 1999
Z H Li D P Liu C C Liang

Inverse polymerase chain reaction (PCR) is a powerful approach for cloning the DNA sequences lying outside the boundaries of known sequences (7). This approach can be used to determine the integration site of a virus or a transposon (2,4). The procedure described by Triglia (7) can be directly used to amplify the flanking sequence in a plasmid or in a bacterial genome. However, it has been diff...

Journal: :Journal of Applied Polymer Science 1995

Journal: :Proceedings of the National Academy of Sciences of the United States of America 2013
Lin Sun Adam F Johnson Ryan C Donohue Jilong Li Jianlin Cheng James A Birchler

Dosage compensation, the equalized X chromosome gene expression between males and females in Drosophila, has also been found in triple X metafemales. Inverse dosage effects, produced by genomic imbalance, are believed to account for this modulated expression, but they have not been studied on a global level. Here, we show a global expression comparison of metafemales (XXX; AA) with normal femal...

Journal: :Blood 1998
M Nakayama M Wada T Harada J Nagayama H Kusaba K Ohshima M Kozuru H Komatsu R Ueda M Kuwano

Selection of human cells for resistance to vincristine or doxorubicin often induces overexpression of the multidrug resistance 1 gene (MDR1), which encodes the cell surface P-glycoprotein, as a result of gene amplification or transcriptional activation. Moreover, overexpression of the MDR1 gene has been shown to be associated closely with clinical outcome in various hematological malignancies, ...

Journal: :Blood 1998
J N Winter J Andersen J C Reed S Krajewski D Variakojis K D Bauer R I Fisher L I Gordon M M Oken S Jiang D Jeffries P Domer

An inverse relationship between BCL-2 expression and cell cycle transition has been suggested by recent studies in murine models. To investigate the clinical relevance of these laboratory studies, a group of 116 paraffin-embedded non-Hodgkin's lymphoma (NHL) biopsy specimens (Working Formulation Groups D-H, and J) from a cooperative group study of cellular DNA content were analyzed for the 14;1...

2016
Vânia Ames Schommer Airton Tetelbom Stein Aline Marcadenti Estefania Inez Wittke André Luís Câmara Galvão Guido Bernardo Aranha Rosito

Objective: To evaluate the association between obesity and levels of high-sensitivity C-reactive protein (hs-CRP) in patients with heart failure admitted to a tertiary hospital. Methods: Cross-sectional study with a consecutive sampling of hospitalized patients with heart failure. Sociodemographic and clinical data were collected, and the nutritional status was assessed through indicators suc...

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