I was interested to read Rodríguez et al. (2012) on PCR to detect verrucosin producing fungi in food and cultures. However, their interpretation regarding mutated strains and internal amplification controls (IAC) appears to be incorrect, although they referred to our papers on the issue. It is worth restating to the wider scientific community that fungi may be mutated by self produced mutagens ...

Since the introduction of the Sarbanes-Oxley (SOX) Act in 2002, companies have begun to place more emphasis on information technology (IT) internal controls. IT internal controls are policies that provide assurance that technical systems operate as intended, provide reliable data, and comply with regulations. Research suggests that firms with strong internal controls perform better than those w...

Mitochondrial DNA (mtDNA) is a small but significant part of the human genome, whose applicability potential has gradually increased with advent massively parallel sequencing (MPS) technology. Knowledge particular workflow, equipment, and reagents used, along extensive usage negative controls to monitor all preparation steps constitute prerequisites for confident reporting results. In this stud...

In the 19 years since the first descriptions of the PCR (1 ), nucleic acid amplification methods have made the transition from research to clinical laboratories. Molecular diagnostics are now firmly established as part of laboratory medicine, with applications in genetics, oncology, pharmacology, and infectious disease. Routine diagnostic applications of these methods have been made possible by...

Regulatory compliance requirements in the area of Internal Controls such as Sarbanes Oxley Act force enterprises to identify, shape and document their business processes. In this context enterprises require mechanisms to ensure that their business processes implement and fulfill compliance requirements independently from business level requirements. In this paper we present a novel approach for...

A new approach for an internally controlled PCR was developed using a custom-synthesized oligonucleotide as the internal control. Three different PCR setups demonstrated the usefulness of this approach: (i) the addition of the respective internal control to samples containing ssDNA virus Parvo B19; (ii) the co-extraction of plasma samples and the respective internal control for the detection of...