background: leishmaniasis is a tropical disease that is endemic in some areas of iran, including east azerbaijan . ifn-γ is one of the cytokines that triggers cell-mediated immunity, thus initiating elimination of the infection. this case-control study was performed to investigate the association between the polymorphism of the ifn-γ gene at the +874a/t locus and visceral leishmaniasis (vl). me...

conclusions here we review the clinical applications, advantages, and limitations of the tuberculin skin test and interferon-gamma release assays and provide an overview of the most recent systematic reviews conducted for the comparison of these tests. context it has been estimated that one-third of the world’s population are suffering from latent tuberculosis infection (ltbi). in order to cont...

BACKGROUND Although the onset of anemia during infectious disease is commonly correlated with production of inflammatory cytokines, the mechanisms by which cytokines induce anemia are poorly defined. This study focused on the mechanism research. METHODS Different types of mice were infected perorally with Toxoplasma gondii strain ME49. At the indicated times, samples from each mouse were harv...

Combining computational and experimental tools, we present a new strategy for designing high affinity variants of a binding protein. The affinity is increased by mutating residues not at the interface, but at positions lining internal cavities of one of the interacting molecules. Filling the cavities lowers flexibility of the binding protein, possibly reducing entropic penalty of binding. The a...

tuberculosis pericarditis as a potentially fatal complication of tuberculosis requires effective diagnosis and treatment. we evaluated the efficacy of interferon-gamma (ifn-gamma) and adenosine deaminase (ada) for diagnosing tuberculosis pericarditis in a cohort of iranian patients presenting with pericarditis. we enrolled 38 patients with presentation of pericarditis. all patients underwent di...

Immunization of mice with heat shock protein 60 from Histoplasma capsulatum or a polypeptide from the protein designated F3 confers protection. Vbeta8.1/8.2+ T cells are critically important for the protective efficacy of this antigen. The production of interleukin-10 and gamma interferon following vaccination is essential for efficacy. In this study, we sought to determine whether the absence ...

In this cross-sectional study, we analysed serum concentrations of soluble markers of cellular immune activation, namely, interferon-gamma, neopterin (a product of activated macrophages), soluble interleukin-2 receptor, and soluble CD8, in 25 patients with congestive heart failure. Ten (40%) patients showed increased concentrations (i.e. above the reference ranges of healthy controls) of neopte...

Interleukin (IL)-12 synergizes with other cytokines to stimulate the proliferation and differentiation of early hematopoietic progenitors in vitro. However, in vivo administration of IL-12 decreases peripheral blood counts and bone marrow hematopoiesis. Here, we used interferon (IFN) gamma receptor-deficient (IFN gamma R-/-) mice to investigate whether the in vivo inhibition of hematopoiesis by...

background: cytokines and specially interferon-gamma (ifn-g) are largely responsible for the regulation of the protective im­mune response against mycobacterial infections. several studies have clarified the importance of common variants of ifn- g gene regarding the susceptibility to tuberculosis. bacille calmette-guérin (bcg) vaccine that is used to prevent se­vere forms of tuberculosis could ...

Natural killer (NK) cells have therapeutic potential for cancer due to their capacity for targeting tumor cells without prior sensitization. Our laboratory has developed an NK cell expansion protocol that generates large quantities of NK cells for therapeutic infusion that secret 20 times the amount of interferon gamma (IFNγ) than resting NK cells. IFNγ can upregulate major histocompatibility c...