Pure nucleotide precursor pools are a prerequisite for high-fidelity DNA replication and the suppression of mutagenesis and carcinogenesis. ITPases are nucleoside triphosphate pyrophosphatases that clean the precursor pools of the non-canonical triphosphates of inosine and xanthine. The precise role of the human ITPase, encoded by the ITPA gene, is not clearly defined. ITPA is clinically import...

Inorganic pyrophosphatase was purified BOO-fold from human erythrocytes by a procedure involving complete hemolysis of the cells, removal of hemoglobin, ammonium sulfate fractionation, diethylaminoethyl cellulose column chromatography, and gel filtration. Magnesium chloride and 2-mercaptoethanol stabilized the activity. The procedure yielded an enzyme with an optimal pH of 7.7, and an apparent ...

Inosine, an endogenous purine, is the first metabolite of adenosine in a reaction catalyzed by adenosine deaminase. This study aimed to investigate the antinociceptive effects of inosine against several models of pain in mice and rats. In mice, inosine given by systemic or central routes inhibited acetic acid-induced nociception. Furthermore, inosine also decreased the late phase of formalin-in...

The ATP-induced enhancement of the intrinsic fluorescence of myosin and heavy meromyosin (HMM) that persists during the steady state of hydrolysis has been investigated. To compare the substrateinduced changes in fluorescence with those in the electron spin resonance spectrum of the spin-labeled enzyme, we studied the influence of temperature, pH, and ionic strength, as well as the effect of ch...

background: the mitochondria are an important source of adenosine triphosphate (atp) production in pre-implantation embryo. therefore, the objective of this study was to investigate the effect of vitrification and in vitro culture of mouse embryos on their mitochondrial distribution and atp content. methods: the embryos at 2-pn, 4-cell and blastocyst stages were collected from the oviduct of st...

The formation of acetyl phosphate and adenosine diphosphate (ADP) from adenosine triphosphate (ATP) and acetate was first recognized by F. Lipmann (J. Biol. Chem. 155:55, 1944) in extracts of Lactobacillus delbrueckii and later by E. R. Stadtman and H. A. Barker (J. Biol. Chem. 184:769, 1950) in Clostridium kluyveri. I. C. Rose et al. (J. Biol. Chem. 211:737, 1954) purified the acetokinase of E...

The presence of 10 microM dipyridamole in incubation media of L1210/C2 cells decreased initial rates of zero-trans influx of formycin B (FB, 50 microM), a poorly metabolized inosine analogue, from 4.84 pmol/microliters cell water/s to 0.87 pmol/microliter cell water/s. However, after a 5-min interval of uptake, free FB levels in dipyridamole-treated cells were 165 pmol/microliters cell water, 2...

2 Abbreviations used are: IMP, inosine monophosphate; AMP, adenosine monophosphate; XMP, xanthosine monophosphate; 6-MP, 6-mercaptopurine; ATP, adenosine triphosphate; 6-thio IMP, 6-thioinosinate; GTP, guanosine triphosphate; NAD, nicotinamide adenine dinucleotide; DPN-diphosphopyridine nucleotide ; SAICAR, succinoaminoimidazolecarboxamide ribo nucleotide; CMC-carboxymethyl cellulose; Tris, tri...

OBJECTIVE Inosine is a naturally occurring nucleoside degraded from adenosine. Recent studies have demonstrated that inosine has potent immunomodulatory and neuroprotective effects. In the present study, we further investigated the inhibitory effects of inosine on platelet activation in vitro and in vivo, as well as in attenuating middle cerebral artery occlusion (MCAO)-induced focal cerebral i...