Accumulation of calcium in the mitochondria of rat liver parenchymal cells at 16 and 24 hours after poisoning with carbon tetrachloride is associated with an increase in amount of liver inorganic phosphate, the persistence of mitochondrial adenosine triphosphatase activity, and the formation of electron-opaque intramitochondrial masses in cells with increased calcium contents. These masses, whi...

Levels of the high-energy phosphate-containing compounds, ATP and creatine phosphate, and of inorganic phosphate (Pi) were measured as a function of position in the cardiac cycle. Measurements were made on isolated, perfused, working rat hearts through the use of gated 31P nuclear magnetic resonance spectroscopy. Levels of ATP and creatine phosphate were found to vary during the cardiac cycle a...

We report a selective method to make functional bio-inorganic materials by mineralizing cobalt-phosphate in the presence of His-tagged enzymes. We have demonstrated that the His-tag drives the biomineralization forming sponge-like structures where both inorganic and biological elements co-localize. The bio-inorganic catalysts were re-used for several redox reaction cycles demonstrating their po...

Highly purified commercial alkaline phosphatase preparations from Escherichia coli have been shown to catalyze the transfer of a phosphoryl group of inorganic pyrophosphate to the hydroxyl group attached to carbon atom 6 of glucose. Phosphotransferase activity also was detected with relatively crude chicken intestinal alkaline phosphatase preparations. The E. coli enzyme was further characteriz...

Certain phosphocreatine preparations contain a contaminant that inhibits phosphofructokinase and pyruvate kinase assays. The contaminant can be separated from phosphocreatine by anion exchange chromatography. After appropriate purification, phosphocreatine has no effect on phosphofructokinase or pyruvate kinase; thus, there is no evidence that it serves muscle as a regulator of these enzymes. A...

We describe how concentrations of chloride, urea, inorganic phosphate, and creatinine in urine can be measured directly, without manual sample dilution, in a discrete analyzer (the Technicon "RA-XT"). These methods were accurate for concentrations of chloride up to 280 mmol/L, urea up to 500 mmol/L, inorganic phosphate up to 50 mmol/L, and creatinine up to 30 mmol/L. CVs are less than 3% nd res...

This report gives a comparison of the specificity of phosphate translocators in various plastids. Whereas the phosphate translocator of the C(3) plant spinach mediates a counter exchange between inorganic phosphate, dihydroxyacetone phosphate, and 3-phosphoglycerate, the phosphate translocators in chloroplasts from C(4) and CAM plants transport phosphoenolpyruvate in addition to the above menti...

Although exponential growth of Bacillus subtilis 168 in a phosphate-limited medium halted with the exhaustion of inorganic phosphate, the bacteria continued to grow at a slower rate for a further 3 to 4 h at 37 degrees C. This postexponential growth in the absence of an exogenous phosphate supply was accompanied by a loss of teichoic acid from the cell walls of the bacteria. Quantitative analys...

Highly purified commercial alkaline phosphatase preparations from Escherichia coli have been shown to catalyze the transfer of a phosphoryl group of inorganic pyrophosphate to the hydroxyl group attached to carbon atom 6 of glucose. Phosphotransferase activity also was detected with relatively crude chicken intestinal alkaline phosphatase preparations. The E. coli enzyme was further characteriz...