نتایج جستجو برای: indirect immunofluorescence

تعداد نتایج: 102331  

2017
In Young Yoo Jong Won Oh Hoon-Suk Cha Eun-Mi Koh Eun-Suk Kang

BACKGROUND The gold standard for antinuclear antibody (ANA) screening is the indirect immunofluorescence (IIF) assay with human epithelial cells (HEp-2). However, a number of substantial disadvantages of manual IIF assays have highlighted the need for the automation and standardization of fluorescent ANA (FANA) testing. We evaluated the performance of EUROPattern Suite (Euroimmun AG, Germany), ...

2012
Tania Caroline Castro Daniela Petry Piotto

Purpose Indirect immunofluorescence (IIF) in Hep-2 cells is the standard screening test for antinuclear antibodies (ANA). Advances in this methodology have brought up a considerable increase in sensitivity and consequently a decrease in its specificity. This has resulted in an increasing number of positive tests in apparently healthy subjects. In particular, autoantibodies associated with the d...

2012
Annika Willitzki Rico Hiemann Vanessa Peters Ulrich Sack Peter Schierack Stefan Rödiger Ursula Anderer Karsten Conrad Dimitrios P. Bogdanos Dirk Reinhold Dirk Roggenbuck

Antibody assessment is an essential part in the serological diagnosis of autoimmune diseases. However, different diagnostic strategies have been proposed for the work up of sera in particular from patients with systemic autoimmune rheumatic disease (SARD). In general, screening for SARD-associated antibodies by indirect immunofluorescence (IIF) is followed by confirmatory testing covering diffe...

2013
Makoto Miyara Roger Albesa Jean-Luc Charuel Mohamed El Amri Marvin J. Fritzler Pascale Ghillani-Dalbin Zahir Amoura Lucile Musset Michael Mahler

OBJECTIVE To analyze the clinical value of anti-DFS70 antibodies in a cohort of patients undergoing routine antinuclear antibodies (ANAs) testing. METHODS Sera with a dense fine speckled (DFS) indirect immunofluorescence (IIF) pattern from 100 consecutive patients and 100 patients with other IIF patterns were tested for anti-DFS70 antibodies by a novel chemiluminescence immunoassay (CIA) and ...

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