In situ optical meters are widely used to estimate leaf chlorophyll concentration, but non-uniform chlorophyll distribution causes the opticalmeasurement to vary widely among species for the same chlorophyllconcentration. Over 30 studies have sought to quantify the in situ/in vitro (optical/absolute) relationship, but neither chlorophyll extraction normeasurement techniques for ...

A central reaction of chlorophyll breakdown, porphyrin ring opening of pheophorbide a to the primary fluorescent chlorophyll catabolite (pFCC), requires pheophorbide a oxygenase (PAO) and red chlorophyll catabolite reductase (RCCR), with red chlorophyll catabolite (RCC) as a presumably PAO-bound intermediate. In subsequent steps, pFCC is converted to different fluorescent chlorophyll catabolite...

Chlorophyll-a was regarded as the important indicator to describe the marine primary production because the chlorophyll a content of phytoplankton in the ocean is related to its photosynthesis production.The concentration of chlorophyll a is also the major parameter to evaluate marine water quality, organic pollution and detect the fishing ground, and the temporal and spatial variation of chlor...

Symbioses with the dinoflagellate Symbiodinium form the foundation of tropical coral reef communities. Symbiodinium photosynthesis fuels the growth of an array of marine invertebrates, including cnidarians such as scleractinian corals and octocorals (e.g., gorgonian and soft corals). Studies examining the symbioses between Caribbean gorgonian corals and Symbiodinium are sparse, even though gorg...

A spectroscopic characterization is presented of the minor photosystem II chlorophyll a/b-binding protein CP29 (or the Lhcb4 protein) from spinach, prepared by a modified form of a published protocol [Henrysson, T., Schroder, W. P., Spangfort, M. & Akerlund, H.-E. (1989) Biochim. Biophys. Acta 977, 301-308]. The isolation procedure represents a quicker, cheaper means of isolating this minor ant...

Drought stress is one of the major environmental constraints to crop plants including wheat worldwide. Synthetic hexaploid can act as a vehicle for improving crop tolerance against biotic and abiotic stresses. Doubled haploid population consisting of one hundred and forty individuals derived from cross of Opata and SH223 was used in the present study to identify genomic regions associated with ...

In saturating light radish seedlings grown in high-light growth conditions (90 W • n r 2) possess a much higher photosynthetic capacity on a chlorophyll and leaf area basis than the low-light grown plants (10 W • m-2). The higher C 0 2-fixation rate of HL-plants is due to the presence of HL-chloroplasts which possess a different ultrastructure and also different levels of the individual chlorop...

Delayed light emission (DLE) from aggregated chlorophyll is used to probe energy transfer between aggre­ gated chlorophyll and ß-carotene. Preilluminated ßcarotene when injected into a dark chamber containing aggregated chlorophyll, induces DLE from aggregated chlorophyll. If the dark chamber contains only monomeric chlorophyll, there is no DLE. The intensity of DLE is de­ pendent on the time o...

Chlorophyll b is found in photosynthetic prokaryotes and primary and secondary endosymbionts, although their light-harvesting systems are quite different. Chlorophyll b is synthesized from chlorophyll a by chlorophyllide a oxygenase (CAO), which is a Rieske-mononuclear iron oxygenase. Comparison of the amino acid sequences of CAO among photosynthetic organisms elucidated changes in the domain s...

The reaction of several plant chlorophyll-protein complexes with NaBH, has been studied by absorption spectroscopy. In all the complexes studied, chlorophyll b is more reactive than Chl a , due to preferential reaction of its formyl substituent at C-7. The complexes also show large variations in reactivity towards NaBH, and the order of reactivity is: LHCI > PSI1 complex > LHCII > PSI > P700 (i...