Background In vitro organogenesis is one of the key techniques associated with genetic transformation, as it determines the successful regeneration of transgenic plants after co-cultivation with bacteria. Therefore, the development of efficient regeneration protocols is the most critical step in developing genetic transformation. Protocols have been developed for several species of eucalyptus. ...

Background: In the last few years, a marked decrease in male fertility has been reported. Environmental factors were recently suspected for this problem. The misuse of medicines especially antibiotics are among environmental factors that can affect reproductive system. Ciprofloxacin is a synthetic antibacterial agent belonging to the family of fluoroquinolones with a very broad spectrum effect ...

A protocol was developed for high frequency in vitro multiplication of an ornamental orchid, Phalaenopsis amabilis (L.) Blume var. Jawa, using plant growth regulators (PGRs). Protocorm-like bodies (PLBs), as explants were cultured on Murashige and Skoog (MS) medium fortified with various concentrations of kinetin (KIN) and indole-3-butyric acid (IBA), either individually or in...

The success of plant tissue cultures is influenced by plant growth regulators, nutrient supply, and the carbon source in the medium. We investigated the most suitable carbon source and concentration for the in vitro rooting of Rehmannia glutinosa. Stem segments of R. glutinosa were cultured in vitro with 30 g/L of seven different carbon sources (sucrose, dextrose, mannose, glucose, sucrose, gal...

Background: While assisted reproductive techniques (ARTs) are considered to be safe, many concerns exist about the safety of these techniques. Two important and indispensable parts of these techniques are in vitro culture and vitrification. While much progress has been made in developing culture conditions, it remains suboptimal. In order to investigate the effect of culture medium on in vitro ...

in vitro multiple shoot regeneration of solanum nigrum l., an indian medicinal plant was accomplished on ms medium utilizing shoot tip and nodal explants. direct multiple shoots differentiated within 6 weeks when explants were cultured on ms medium containing bap (1.0-5.0 mg/l) and kin (1.0-5.0 mg/l) individually. among various concentrations of cytokinins tested, maximum number of multiple sho...