oocyte maturation and embryo development are controlled by intra-ovarian factors such as steroid hormones. progesterone (p4) exists in the follicular fluid that contributes to normal mammalian ovarian function and has several critical functions during embryo development and implantation, including endometrial receptivity, embryonic survival during gestation and transformation of the endometrial...

Follicular fluid (FF) provides a very important microenvironment for the development of oocyte maturation, ovulation and fertilization. The objective of this study was to determine the effect of different charged groups of cow follicular fluid proteins on in vitro oocyte maturation (IVM) when added to cultured maturation medium. The follicular fluid were obtained from dominant follicle and prec...

Background: The objectives of this study were: a) to observe the influence of cumulus investment expansion on the nuclear chromatin configuration of canine oocytes matured in vitro; b) to examine the relationship between cumulus cell (CC) expansion and its morphology after in vitro maturation (IVM); c) to ascertain the influence of in vivo serum progesterone (SP) concentrations of ovary donors ...

Background: Cryopreservation of oocytes, which is an interesting procedure to conserve female gametes, is an essential part of reproductive biotechnology. The objective of the present study was to investigate the effects of cumulus cells on survival,in vitro maturation,fertilization and subsequent developmental capacity of mouse germinal vesicle stage oocytes during vitrification. Materials and...

Background: Melatonin has been recognised as effective reactive oxygen species (ROS) scavenger. antioxident melatonin effects has been expressed in management of cancer, alzheimer, diabet diseases. This study designed to determine the effect of melatonin on in vitro oocytes maturation and fertilization and cleavage and blastocyst development rate of mouse. Materials and Methods: Cumulus - oocyt...

introduction: in vitro maturation and development of immature oocytes, as an artificial reproduction technique, is useful especially in women who are affected by cancer and polycystic ovary syndrome. despite using many types of in vitro media, an appropriate environment has not been reported yet. present study was designed to assess the effect of heated human follicular fluid (hhff), which is s...