Lenses from all vertebrate classes were used for preparing antisera in rabbits. By immunoelectrophoresis, 9 to 10 precipitin lines were found in all homologous reactions. The number of lens components shared by different vertebrates depended on their relative position in the evolutionary scale. The absorption of any given antilens serum with lenses from another class removed antibodies against ...

The group R streptococcal group antigen has been shown to be a polysaccharide located at the surface of the cell wall of the organism. The antigen was extracted from cell walls in 0.05 n HCl or 5% trichloracetic acid at 100 C, from whole cells at room temperature in 0.85% NaCl or 0.1 m acetate (pH 5.0), and by sonic oscillation. The antigen is largely destroyed when extracted from whole cells i...

* The use of trade or firm names in this publication is for reader information and does not imply endorsement by the U.S. Department of Agriculture of any product or service. The Forest Products Laboratory is maintained in cooperation with the University of Wisconsin. This article was written and prepared by U.S. Government employees on official time, and it is therefore in the public domain an...

A study has been made of the interaction of specific anti-nitroxide (anti-spin label) IgG antibodies, Fab fragments, complement, and liposomes containing dimyristoylphosphatidylcholine, dipalmitoylphosphatidylcholine, and dipalmitoylphosphatidylcholine plus 0-50 mole % cholesterol, together with various concentrations of a head group spin-labeled phospholipid (0.5-0.01 mole % in the plane of th...

A questionnaire was prepared under the auspices of the Department of Health with the aim of defining the extent and nature of immunocytochemistry use within pathology departments. The questionnaire was circulated to 320 pathology laboratories within the United Kingdom, and a total of 178 replies were received, representing a response rate of 56%. One hundred and thirty eight (78%) of the respon...

Pharmacology of Cyclosporine (Sandimmune) Introduction, J. F. BOREL, F. DI PADOVA, JUNE MASON, VALERIE QUESNIAUX, B. RYFFEL, AND R. WENGER 239 Chemistry, ROLAND M. WENGER 243 Immunochemistry and Monitoring, VALERIE F. J. QUESNIAUX 449 Pharmacological Properties in Vivo, JEAN F. BoI FL 259 Pharmacological Effects on Immune Function: In Vitro Studies, FRANCO E. DI PADOVA.... 373 Cellular Activati...

As assessed by both standard histological staining and immunochemistry, intraperitoneal inoculation of C. burnetii in guinea pigs led to pathologic changes mainly in the liver, whereas intranasal inoculation led to pathologic changes mainly in the lungs. Myocarditis and positive blood cultures were observed only in those animals which received the highest inoculum. We therefore conclude that bo...

The measurement of total haemolytic complement is of diagnostic value in the rheumatic diseases. A method which is easier to perform than that of Kabat and Mayer (Experimental Immunochemistry, 2nd ed., Thomas, Springfield, Ill.) is described. A unit based on the mean value of the complement activities of normal sera is proposed to replace CH50 units. Other steps by which standardisation of the ...