The ability of Legionella pneumophila to act as a recipient of IncP and IncQ plasmids in matings with Escherichia coli varies widely from strain to strain. We found that the low efficiency of mating of the Philadelphia-1 strain is due to a type II restriction-modification system, and we isolated and characterized a Philadelphia-1 mutant that lacks the restriction enzyme activity.

this study was carried out to determine the potential of rumen microorganisms in samples taken from slaughterhouse to produce fibrolytic enzymes and to compare different methods for extraction, concentration and conservation of them.  according to the results, the average specific activity of cellulase and xylanase were 7.5 and 16.5 u/mg protein respectively. the application of homogenizing and...

We study how crowding affects the activity and catalysis-enhanced diffusion of enzymes passive tracers by employing a fluctuating-dumbbell model conformation-changing enzymes.

Introduction The enzymes tryptophan 2,3-dioxygenase (TDO) and indoleamine 2,3-dioxygenase (IDO1) catalyse oxidation of the essential amino acid tryptophan (Trp) leading to the formation of immunosuppressive kynurenine (Kyn) pathway metabolites that dampen the immune response in the tumour microenvironment. Both IDO1 and TDO have been shown to be up-regulated in a variety of cancers and blockade...

The Cytochromes P450 are heme-thiolate enzymes that carry out selective oxyfunctionalization of a wide range substrates using molecular dioxygen and two reducing equivalents provided by redox partner enzymes. Alternative approaches to activate have received recent attention in an attempt circumvent the use partners cofactors. In particular, light-harvesting units has proven useful triggering ac...

Nanoparticles affect the immune system as they may interact directly with immune cells and activate them. However, it is possible that nanoparticles also interact with released cytokines and immunologically active enzymes. To test this hypothesis, the activity of myeloperoxidase released from activated neutrophils was measured in the presence of nanoparticles with different chemistry and size. ...

The production of β-maltooligosaccharides of capsaicin was investigated using Lactobacillus delbrueckii and cyclodextrin glucanotransferase (CGTase) as biocatalysts. The cells of L. delbrueckii glucosylated capsaicin to give its β-glucoside. The β-glucoside of capsaicin was converted into the corresponding β-maltoside and β-maltotrioside by CGTase. On the other hand, β-melibioside and β-isomalt...