As a consequence of intense industrialization in the last few decades, amount agro-industrial wastes has increasing, where new forms valorization are crucial. In this work, five residual biomasses from Maranhão (Brazil) were investigated as supports for immobilization lipase Thermomyces lanuginosus (TLL). The biocatalysts BM-TLL (babaçu mesocarp) and RH-TLL (rice husk) showed efficiencies >9...

presently there are a number of fine and sensitive methods for determining chemical or biochemical substance among which bio electrodes represent the most recent development. bioelectrodes consist of biologically active materials (e.g enzyme, antibody, whole cell or cell fragments) held in close proximity to a suitable electrochemical transducer that sense specific electroactive enzyme substrat...

BACKGROUND For industrial bioconversion processes, the utilization of surface-displayed lipase in the form of whole-cell biocatalysts is more advantageous, because the enzymes are displayed on the cell surface spontaneously, regarded as immobilized enzymes. RESULTS Two Pichia pastoris cell surface display vectors based on the flocculation functional domain of FLO with its own secretion signal...

Biocatalytic syntheses often require unfavorable conditions, which can adversely affect enzyme stability. Consequently, improving the stability of biocatalysts is needed, and this achieved by immobilization. In study, we aimed to compare soluble immobilized transaminases from different species. A cysteine in a consensus sequence was converted single aldehyde formylglycine-generating for directe...

Biocatalysis has emerged as a great addition to traditional chemical processes for production of bulk chemicals and pharmaceuticals. To overcome the limitations of naturally occurring enzymes, directed evolution has become the most important tool for improving critical traits of biocatalysts such as thermostability, activity, selectivity, and tolerance towards organic solvents for industrial ap...

The use of enzymes as industrial biocatalysts is currently a solution for many problems of modern organic chemistry, which tries to carry out the most complex reactions under the rules of green chemistry [1]. In this context, enzyme immobilization is a critical point for the implementation of many processes [2]. This technique has been developed to obtain a heterogeneous biocatalyst, and thereb...

Currently there is a significant amount of information about the way biocatalysts from animals detoxicate and bioactive drugs of abuse. In some cases, biotransformation data concerning drugs of abuse obtained from animal systems are analogous to the human situation, but in many cases the data are not. Clearly, significant work needs to be done with human biocatalysts to define a role in the bio...