The importance of mesenchymal stem cells (MSCs), as adult stem cells (ASCs) able to divide into a variety of different cells is of utmost importance for stem cell researches. In this study, the ability of cartilage extract to induce differentiation of rat derived omentum tissue MSCs (rOT-MSCs) into chondrocyte cells (CCs) was investigated. After isolation of rOT-MSCs, they were co-cultured with...

Background: The breast cancer is the most prevalent cancer among women, on the other hand absence of myoepithelial cells play a pivotal role in pathogenesis of this cancer. Thus we aimed to investigate the possible abilities of the molecular assay technique to find a relationship between mammary serine protease inhibitor (Maspin) gene expression possibly secreted by my...

the present  study  was carried out to investigate the polymorphism of intron 3 to exon 3 of prolactin gene containing 24 bpindel at nucleotide position (np)  358 and its association with some reproductive traits in japanese quail. these traits consisted of weight (wsm) and age at sexual maturity (asm), mean egg weight at 2nd, 4th, 6th, and 2-6th weeks (mew), and the number of eggs during the 2...

We tested the hypothesis that central angiotensin II (ANG II) administration would activate splenic sympathetic nerve discharge (SND), which in turn would alter splenic cytokine gene expression. Experiments were completed in sinoaortic nerve-lesioned, urethane-chloralose-anesthetized, splenic nerve-intact (splenic-intact) and splenic nerve-lesioned (splenic-denervated) Sprague-Dawley rats. Sple...

Abbreviations: attE: attacin E gene ELISA: Enzyme Linked Immunosorbent Assay gusA: β-glucuronidase gene GUS: glucuronidase nos: nopaline synthase gene nptII: neomycin phosphotransferase II gene NPTII: neomycin phosphotransferase II protein PCR: polymerase chain reaction PTGS: post-transcriptional gene silencing RNA: ribonucleic acid RT-PCR: reverse transcription PCR T-DNA: transfer DNA TGS: tra...

background: epidemiological investigations on toxoplasma gondii infection have found a significant association between human toxoplasmosis and consumption of raw or undercooked meat. the present study aimed to characterize genotypes of t. gondii in 20 cattle, 40 sheep, 15 goats and 16 pigs from the north of portugal. methods: nested pcr amplified the surface antigen 2 (sag2) gene. sequencing an...

Accessory gene regulator (agr locus) is a global regulator of many virulence gene expressions inStaphylococcus aureus. Four interference classes related to genetic polymorphisms in the agr locus have sofar been described. In the present study, the agr locus specificity groups were studied within a total of 43 S.aureus isolates which were isolated from ewes with mastitis in three regions in the ...

Background: Cadmium is one of the heavy metals with harmful effects on different body organs and systems. The aim of this study was to investigate the harmful effects of cadmium, as a heavy metal, on the histological structure of bone and the expression of osteocalcin gene. Methods: Forty chickens were obtained, anesthetized and their femurs were surgically removed. The real time polymerase ch...

There is no functional annotation for the majority of the several hundreds of receptor-like kinases in plants. A direct way of inferring the function of these proteins is to study the phenotype that results from loss of function mutants such as T-DNA mutant lines. In this research a function (phenotype) to At2g37050 gene that encodes a receptor like kinase in Arabidopsis T-DNA line was...