The nuclear modulation effect in pulsed EPR spectroscopy was used to probe the metal-protein bonds in Cu(II)=transferrin complexes as a function of pH. In preparations in equilibrium with air at pH 4.8 no modulation features other than those attributable to protons were observed, in keeping with conventional EPR studies indicating that at this pH only nonspecific binding takes place (Zweier, J....

Mn(II), Co(II), Ni(II), Cu(II), Zn(II), Cd(II), and Hg(I1) form metallocarbonic anhydrases containing these metal ions at the same binding site. Of this series, only the Zn(I1) and Co(I1) enzymes catalyze the hydration of CO2 and the hydrolysis of p-nitrophenyl acetate. Likewise, only Zn(I1) and Co(I1) induce the metal ion-dependent binding of 3H-acetazolamide at the active center. Acetate, azi...

The reaction between hydrogen peroxide and ferrous EDTA generates an oxidizing intermediate (I1) which is not the hydroxyl radical. It oxidizes ferrocytochrome c and also reacts with hydrogen peroxide (k5 = 3.2 X 10(3) M-1 S-1) to form a second oxidizing transient (I2). I1 is not scavenged by t-butyl alcohol whereas I2 is. I1 is found to be significantly less reactive than the hydroxyl radical ...

Bovine trophoblast protein-1 (bTP-1) is a Type I interferon secreted by the bovine trophoblast from about Day 15 of pregnancy. It is not known whether bTP-1 has functional properties in common with other interferons. The aim of the present study was to determine whether bTP-1 inhibits proliferation of lymphocytes induced by mitogens, mixed lymphocyte cultures (MLC) and interleukin-2 (IL-2) and,...

Mathematical modelling provides a comprehensive understanding of the charge transport process inside solar cells and determines various elements affecting their performance. In present paper, proposed CH3NH3Pb(I1−xBrx)3-based perovskite consisting Cd1−xZnxS as electron layer (ETL) have been mathematically simulated using SCAPS-1D simulator. Hole layers (HTLs) poly(3-hexylthiophene) (P3HT), Cu2O...

the synthesis and single-crystal x-ray structure determination of the title compound (dinitrato) (1,4,8,11 -tetra-azaandecane) lead(ii) is described. crystals are monoclinic, p2 /ca= 10.271 (2)?,b= 13.524(4) ?,c= 11.298(6) ?,?=94.68(3),2=4,r,rw, 0.036 and 0.041, respectively. the lead atom is eight-coordinate [pb-n, 2.44 (2) - 2.55 (1)], [pb-0,2.87 (1) - 3.06 (2)].

We discuss the two-loop integrals necessary for evaluating massless 2 → 2 scattering amplitudes. As a test process, we consider the leading colour two-loop contribution to q ¯ q → q ′ ¯ q ′. We show that for physical scattering processes the two Smirnov-Veretin planar box graphs I1 and I2 are accompanied by factors of 1/(D − 4) thereby necessitating a knowledge of both I1 and I2 to O(ǫ). Using ...

Molecular dynamics simulations, computational alanine scanning and sequence analysis were used to investigate the structural properties of the Galpha(i1)/GoLoco peptide complex. Using these methodologies, binding of the GoLoco motif peptide to the Galpha(i1) subunit was found to restrict the relative movement of the helical and catalytic domains in the Galpha(i1) subunit, which is in agreement ...