BACKGROUND AND PURPOSE Hypoxia, or ischemia, is a common cause of neurological deficits in the elderly. This study elucidated the mechanisms underlying ischemia-induced brain injury that results in neurological sequelae. METHODS Cerebral ischemia was induced in male Sprague-Dawley rats by transient ligation of the left carotid artery followed by 60 min of hypoxia. A two-dimensional differenti...

Previously we showed that Protein kinase A (PKA) activated in hypoxia and myocardial ischemia/reperfusion mediates phosphorylation of subunits I, IVi1 and Vb of cytochrome c oxidase. However, the mechanism of activation of the kinase under hypoxia remains unclear. It is also unclear if hypoxic stress activated PKA is different from the cAMP dependent mitochondrial PKA activity reported under no...

Preconditioning with brief ischemia before a sustained period of ischemia reduces infarct size in the perfused heart. A cultured chick ventricular myocyte model was developed to investigate the role of adenosine receptor subtypes in cardiac preconditioning. Brief hypoxic exposure, termed preconditioning hypoxia, prior to prolonged hypoxia, protected myocytes against injury induced by the prolon...

Purpose Postcardiac arrest syndrome (PCAS) shares many features with sepsis including plasma cytokine elevation with dysregulation of cytokine production, and the presence of endotoxin in plasma. PCAS is closely related to ischemia-reperfusion injury. During ischemia-reperfusion injury, neutrophil, which is the first line of innate immunity, plays a major role. In this study, we investigated th...

The role of angiogenesis inhibition in the antitumor activity of recombinant murine interleukin 12 (rmIL-12) was studied in K1735 murine melanomas, the growth of which is rapidly and markedly suppressed by rmIL-12 treatment. On the basis of the prediction that tumor ischemia should result from therapeutic angiogenesis inhibition, tumor cell hypoxia was evaluated as a marker of ischemia using th...

Activation of acetylcholine receptors (AChRs) exerted cardioprotection against ischemia/reperfusion (I/R) injury. However, the mechanisms underlying its are not fully understood. We investigated effects hypoxia/reoxygenation (H/R), condition that mimics an in vivo I/R, presence and absence AChRs agonists cardiomyoblast H9c2 cells. The cells (104 cells/well) were incubated hypoxic chamber with i...

Purpose: to evaluate the topical use effect of angiotensin-converting enzyme (ACE) inhibitor (iACE) Enalaprilat in eye drops on blood flow volume, hypoxia degree, ACE activity, and total antioxidant activity (TAA) aqueous humor rabbits with experimental ocular ischemia. Material methods . Transient ischemia 30 Chinchilla was induced by subconjunctivally injecting 0.2 ml 1 % Phenylephrine. Ocula...

UNLABELLED Although chronic hypoxia is a claimed myocardial risk factor reducing tolerance to ischemia/reperfusion (I/R), intermittent reoxygenation has beneficial effects and enhances heart tolerance to I/R. AIM OF THE STUDY To test the hypothesis that, by mimicking intermittent reoxygenation, selective inhibition of phosphodiesterase-5 activity improves ischemia tolerance during hypoxia. Ad...