Seven genomic fragments encoding isoforms of tomato (Lycopersicon esculentum) plasma membrane H(+)-ATPase were cloned and characterized. Genomic DNA gel-blot analysis indicated that probes corresponding to LHA1 through LHA7 hybridized to a common set of seven to nine restriction fragments at moderate stringency and to single, distinct fragments at high stringency. RNA gel-blot and polymerase ch...

The development of genetically transformed plants is an elusive landmark in Cannabis sativa L. breeding. Despite its economic interest, at present, protocols for producing transgenic C. are scarce. We studied the ability hypocotyl, cotyledon and meristem explants from six hemp varieties plant regeneration. For this, we firstly evaluated vitro regeneration rates hypocotyls cultured medium withou...

The embryogenic capacity of seven cucumber (Cucumis sativus L.) cultivars was examined by tissue culture of cotyledon, young first-leaf, and internode explants. Somatic embryogenesis frequencies differed significantly among the tested cultivars, and ‘Fushinarimidori’ produced the highest number of embryos from either cotyledons or young first leaves. Cotyledonand first-leaf-derived calluses pro...

of embryogenic frequency 88.9 and 72.23 were recorded for stem explants. However, the greatest embryogenic calli fresh weights 14.68 and 9.65 (mg/jar) were derived from leaf explants. Moreover, growth of somatic embryos was investigated during 7weeks. The highest regeneration percentage was achieved for stem explants compared other explants and recorded 88.88 and 66.66 for directly or indirectl...

A new method for culturing spinal cord slices or explants is presented which entails the use of a commercially available purified collagen, Vitrogen. Vitrogen provides a stable three-dimensional matrix for culturing spinal cord explants which is superior to the conventional method of applying explants to moist dishes coated with rat tail collagen. The use of Vitrogen facilitated the culturing o...

four separate experiments were conducted to study the effects of factors controlling the establishment of explants from two walnut genotypes z60 and z63 (damavand). factors were explant type, sampling time, explant orientation on the growth medium and antioxidants type. the explants were collected from the collection garden of tooyserkan walnuts research station. four explant types (apical meri...

Haptoglobin is a putative adiposity marker because its concentration in blood is increased in obese humans. The present studies examined haptoglobin release by explants of adipose tissue in primary culture. Haptoglobin was released by explants of human visceral and subcutaneous adipose tissue at a nearly linear rate over 48 h. Explants of visceral adipose tissue released more haptoglobin than d...

In the present study, 2, 4-dichlorophenoxyacetic acid (2, 4-D) was assessed individually for its effectiveness to induce somatic embryogenesis in tea (Camellia sinenesis L.). Embryonic axes and cotyledons explants were dissected from the seeds. Explants were cultured on Murashige and Skoog (MS) medium containing 0, 1 and 5 mM 2, 4-D alone for embryonic axes and 0, 1 and 5 mM 2, 4-D along with 0...