Title of Document: DECONSTRUCTING THE POLYCLONAL ANTIBODY RESPONSE TO DENGUE VIRUS Laura Anne VanBlargan, Doctor of Philosophy, 2015 Directed By: Dr. Theodore Pierson, Adjunct Professor, and Dr. Anne Simon, Department of Cell Biology and Molecular Genetics Dengue viruses are mosquito-borne flaviviruses that circulate in nature as four related serotypes (DENV1-4). These emerging pathogens are re...

background: rabies virus (rabv) is a deadly neurotropic virus that causes the disease of rabies in humans and animals. l protein is one of the large structural protein of rabies virus, which displays multiple enzymatic activities, and is required for viral transcription and replication.objectives: a truncated l protein of rabies virus is being cloned, expressed and purified to produce relevant ...

Human cytomegalovirus is shown to be a nonspecific polyclonal B cell activator. The B cell response is independent of virus replication and requires little, if any, T cell help.

Specific monoclonal and polyclonal antibody reagents and a double antigen indirect immunofluorescence microscopy technique were used to visualize coagulation factor V in human bone marrow. Marrow aspirates were smeared directly on glass slides, or washed and cytospun onto glass slides, or processed and plated into a plasma/methylcellulose cell culture system. Morphologically identifiable coloni...

BACKGROUND Nephrin is an essential protein for maintaining the normal structure of the podocyte foot process and the glomerular filtration barrier. To analyse the mechanism of proteinuria and treatment of nephrotic syndrome, we produced and characterized polyclonal anti-human nephrin antibodies using a newly developed genetic immunization technique. METHODS An expression vector with full-leng...

When maize calluses are grown in the presence of the RGD peptide, important morphological changes are observed indicating the presence of a likely RGD-binding receptor. Polyclonal antibodies generated against the human beta1 integrin subunit, the platelet integrin alphaIIbeta3 (P23) and antibodies specific for either the beta3 platelet chain or the alphaIIb polypeptide cross-react with glycopro...

Supplemental Experimental Procedures Reagents and antibodies Reagents were purchased as follows: DRB, wortmannin, NAC, doxycycline, BHA, polybrene and H2O2 were from Sigma, and PD98159 from Calbiochem. Polyclonal anti-phospho-AKT, antiAKT, anti-phospho-S6K, anti-phospho-ATF2, anti-ATF2, anti-phospho-p38, anti-p38, antiphospho-IKKα/β and anti-JNK antibodies were from Cell Signalling. Anti-actin,...

in this study we produced and purified a high titer of specific and high affin¬ity fab fragments of anti-digoxin antibody. immunization of rabbits with a conju¬gate of the cardiac glycoside digoxin, coupled by a periodate oxidation method to the amino group of lysine in bovine serum albumin resulted in the production of this type of high titer digoxin-specific antibodies with exceptionally high...