BACKGROUND We determined the best model available for natural follicle decline in healthy women and used this to calculate the radiosensitivity of the human oocyte. METHODS Ovarian failure was diagnosed in six patients with a median age of 13.2 years (range 12.5-16.0) who were treated with total body irradiation (14.4 Gy) at 11.5 years of age (4.9-15.1). We previously estimated the dose of ra...

Background: The cryopreservation of human oocyte would make a significant contribution to infertility treatment, such as using it for oocyte donation and for patients a bout to lose ovarian function due to surgery or chemotherapy. Despite of using standard freezing straws and cryovials or even open pulled straws, only a few successful pregnancies have been arisen from cryopreserved human oocyte...

Mammalian oocyte activation is mediated by cytosolic calcium (Ca(2+)) oscillations initiated upon delivery of a putative 'sperm factor' by the fertilizing sperm. Previous studies suggest the identity of this sperm factor as the testis-specific phospholipase C-zeta (PLCζ). Recently, a post-acrosomal sheath WW domain-binding protein (PAWP) has been proposed as an alternative sperm factor candidat...

Human (26) Brain ESC/iPSC* FPGC MPGC Oocyte 7 6 5 5 3 AdFront_Wen H1_Lis 10wE1_GuoF 10wE2_GuoF AmpMinus_Okae FcF53yNeun_Lis H9_Lis 11wE1_GuoF 19wE1_GuoF AmpPlus_Okae FcM55yNeun_Lis PBAT_Tang 17wE1_GuoF 19wE2_GuoF Rrbs_GuoHS FCN_Zill Ads_Lis* 7w_Tang 9w_Tang Hs1570_Zeng Ff_Lis* UW160_Gkoun UW165_Gkoun Hs1832_Zeng Imr90_Lis* Mfg12y_Lis Mouse (25) Brain ESC FPGC MPGC Oocyte 8 8 2 3 4 129_Xie 2line...

To expand the availability of stem cell lines suitable for basic research and clinical application, somatic cell nuclear transfer has been proposed and will require human oocyte donation. The recommendations made by the California Institute of Regenerative Medicine advisory committee on oocyte donation are based on peer-reviewed, best practices, and best clinical judgment and are intended to as...

BACKGROUND An alginate-based matrix supports the three-dimensional (3D) architecture of non-human primate follicles and, in the presence of FSH, permits the in vitro development of pre-antral follicles to the small antral stage, including the production of ovarian steroids and paracrine factors. The current study investigated the ability of gonadotrophins, fetuin and oxygen (O₂) to improve prim...

Retinoids are essential for ovarian steroid production and oocyte maturation in mammals. Oocyte competency is known to positively correlate with efficient gap junction intercellular communication (GJIC) among granulosa cells in the cumulus-oocyte complex. Connexin 43 (C x 43) is the main subunit of gap junction channels in human cumulus granulosa cells (CGC) and is regulated by all-trans retino...

BACKGROUND Oocyte retrieval failure following an ovarian hyperstimulation protocol is uncommon in assisted reproductive technology (ART) programs. We analyzed the predictive factors for oocyte retrieval failure following controlled ovarian hyperstimulation (COH) with gonadotropin-releasing hormone (GnRH) agonist and GnRH antagonist protocols in ART programs. METHODS This study was a retrospec...

BACKGROUND Mouse oocytes can be used in conjunction with intracytoplasmic sperm injection (ICSI) as a technique to permit chromosomal analysis of human sperm. However, chromosomes derived both from the human sperm and the mouse oocyte appear simultaneously following ICSI. The present study focused on evaluating whether or not previously enucleated mouse oocytes are usable for the analysis of hu...

is believed to occur during the early stages of gamete fusion, and thus before the sperm tail is incorporated into the oocyte. Thus, our figure represents a specific temporal stage of fertilization and not the entire sequence of events. It would, therefore, be inaccurate and misleading to have shown the sperm tail incorporated into the oocyte at this stage. While we are content with the fact th...